Probe:	Gcm2 probe1
Probe preparation:	labelled with digoxigenin
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		1A

Structure	Level	Pattern	Image	Note
TS15: branchial pouch	Present	Regionally restricted	1B	Expression in a diffuse domain encompassing the 3rd and 2nd pharyngeal pouches.
TS15: 3rd branchial pouch	Present		1B
TS15: 2nd branchial pouch	Weak		1B	Lower level of expression in 2nd pharyngeal pouch

Structure	Level	Pattern	Image	Note
TS17: 3rd branchial pouch	Present	Regionally restricted	1C	Expression restricted to 3rd pharyngeal pouch, most strongly expressed in cranial part of pouch.

Structure	Level	Pattern	Image	Note
TS19: 3rd branchial pouch	Present	Regionally restricted	1D	Expression exclusively in small bilateral domains in the pharyngeal region

Structure	Level	Pattern	Image	Note
TS17: 3rd branchial pouch endoderm	Present	Regionally restricted	1E	Strong expression in a small domain within cranial half of 3rd pharyngeal pouch endoderm.

Structure	Level	Pattern	Image	Note
TS19: thymus/parathyroid primordium	Present	Regionally restricted	1F	Expression confined to dorsal and cranial portions of thymus/parathyroid primordia developing from the 3rd pharyngeal pouches.

Structure	Level	Pattern	Image	Note
TS19: thymus/parathyroid primordium	Present	Regionally restricted	1G	Expression in the organ primordium developing from the 3rd pharyngeal pouch.