Probe:

Sox3 probe1

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present	Widespread	1_Sox3 PS	Expression thoughout epiblast.
TS9: extraembryonic ectoderm	Present	Regionally restricted	1_Sox3 PS	Expression in a band of extraembryonic ectoderm abutting the embryonic extraembryonic boundary.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Regionally restricted	1_Sox3 MS-LS	Embryonic expression restricted to anterior ectoderm.
TS10: extraembryonic component	Present	Regionally restricted	1_Sox3 MS-LS	Extraembryonic expression confined to chorion.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	1_Sox3 LB	Expression spreads into posterior epiblast.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	1_Sox3 HF	Strongest expression in posterior ectoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	1_Sox3 section a	Expression restricted to ectodermal layer; spreads into posterior epiblast.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	1_Sox3 section b	Expression restricted to ectodermal layer; spreads into posterior epiblast.

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	2A Sox3 1-3s	Expression in neurectoderm from the onset of somitogenesis. Dynamic pattern of expression with transcripts receding from streak ectoderm.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	2A Sox3 6s	Expression returns in streak ectoderm.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	2B Sox3 6s	Expression returns in streak ectoderm.
TS12: rhombomere	Present	Regionally restricted	2B Sox3 6s	Expression upregulated in rhombomere 5.

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Present	Regionally restricted	2B Sox3 8-10s	Expression in ectoderm overlying 1st and 2nd branchial arches.

Structure	Level	Pattern	Image	Note
TS14: surface ectoderm	Present	Regionally restricted	2B Sox3 12-14s	Expression in ectoderm overlying first branchial arch.

Structure	Level	Pattern	Image	Note
TS12: surface ectoderm	Present	Regionally restricted	2C c	Expression in a region of surface ectoderm adjacent to the hindbrain.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 3	Present	Regionally restricted	2C f	Expression in rhombomere 3 restricted to ventral half, excluding the floorplate.
TS14: rhombomere 3 floor plate	Absent		2C f	Expression in rhombomere 3 restricted to ventral half, excluding the floorplate.
TS14: neural tube floor plate	Absent		2C f	Expression excluded from floorplate of neural tube and from border between neurectoderm and surface ectoderm (future roofplate).
TS14: neural tube roof plate	Absent		2C f	Expression excluded from floorplate of neural tube and from border between neurectoderm and surface ectoderm (future roofplate).

Structure	Level	Pattern	Image	Note
TS14: rhombomere 5	Present	Regionally restricted	2C i	Expression excluded from floorplate of neural tube and from border between neurectoderm and surface ectoderm (future roofplate).
TS14: rhombomere 5 floor plate	Absent		2C i	Expression in rhombomere 5 restricted to ventral half, excluding the floorplate.
TS14: foregut	Present	Regionally restricted	2C i	Expression in posterior region of foregut.