Probe:	clone 2-2B
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		2A/B
TS17: neural tube	Present	Regionally restricted	2A/B	Expression is ventromedial.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	2G	Expression is outside the ependymal layer.
TS17: head mesenchyme	Present	Regionally restricted	2G	Expression is in the basiooccipital mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present		2H/I
TS20: spinal cord	Strong	Regionally restricted	2H/I	Expression is outside the ependymal layer.

Structure	Level	Pattern	Image	Note
TS22: brain	Present		2L
TS22: spinal cord	Present		2L

Structure	Level	Pattern	Image	Note
TS24: lens	Strong		2N/O
TS24: neural retina	Strong	Regionally restricted	2N/O	Expression is in retinal ganglion layer.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		4A/B	Expression is in the condensations of the handplate.

Structure	Level	Pattern	Image	Note
TS20: hand pre-cartilage condensation	Present	Regionally restricted	4C/D	Expression is in the exterior of phalangeal condensations.

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	4G	Expression is in the exterior of tibial condensations.
TS20: knee	Present		4G

Structure	Level	Pattern	Image	Note
TS22: hindlimb mesenchyme	Present	Regionally restricted	4J/K	Expression is in dense connective tissue mesenchyme and virtual exclusion from cartilagenous condensations.

Structure	Level	Pattern	Image	Note
TS20: cervical vertebral cartilage condensation	Present		4L/M

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present	Regionally restricted	4N/O	Expression is in mesenchyme between chondrifications of vertebral bodies and bases of rib shafts.
TS20: vertebral cartilage condensation	Present	Regionally restricted	4N/O	Expression is in mesenchyme between chondrifications of vertebral bodies and bases of rib shafts.

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present	Regionally restricted	4P/Q	Expression is in mesenchyme between chondrifications of vertebral bodies and bases of rib shafts.
TS20: vertebral cartilage condensation	Present	Regionally restricted	4P/Q	Expression is in mesenchyme between chondrifications of vertebral bodies and bases of rib shafts.

Structure	Level	Pattern	Image	Note
TS17: common atrial chamber cardiac muscle	Present		5A/B

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Present		5E

Structure	Level	Pattern	Image	Note
TS22: tail sclerotome	Present	Regionally restricted	6A/B	Expression in in the loose mesenchyme bands of tail sclerotomes and diminishes in segments undergoing vertebral differentiation.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		6C/D

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	6E/F	Expression is in the basiooccipital region above tongue.

Structure	Level	Pattern	Image	Note
TS22: maturing glomerular tuft	Present		6G/H

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Absent
TS24: skeleton	Absent

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present