Probe:	Optx2 P1
Probe preparation:	Antisense RNA
Assay notes:	The authors did not specify which probe was used for this assay, it is possible that shorter probes corresponding to different regions were used (Optx2 P2 and Optx2 P3 corresponding to MGI:5318105 and MGI:5318106 respectively).

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present	Regionally restricted	1A	Expression is in the most rostral portion of the neural plate.
TS13: surface ectoderm	Present	Regionally restricted	1A	Expression is in the surface ectoderm at the most rostral portion of the neural plate.

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present	Regionally restricted	1B	Expression is in the most rostral portion of the neural plate.
TS13: surface ectoderm	Present	Regionally restricted	1B	Expression is in the surface ectoderm at the most rostral portion of the neural plate.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1C	Expression is ventral.
TS13: optic pit	Present		1C

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1D	Expression is ventral.
TS13: optic pit	Present		1D

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present		1E
TS15: future brain	Present	Regionally restricted	1E	Expression is in the optic chiasm and optic nerve.

Structure	Level	Pattern	Image	Note
TS17: optic cup	Present		1F
TS17: forebrain	Present	Regionally restricted	1F	Expression is ventral at the level of the optic chiasm.

Structure	Level	Pattern	Image	Note
TS17: nasal pit	Present	Regionally restricted	1G	Expression is in the anterior rim of the nasal placode.
TS17: eye	Present		1G
TS17: Rathke's pouch	Present	Regionally restricted	1G	Expression is at the entrance to Rathke's pouch.
TS17: brain	Present	Regionally restricted	1G	Expression is at the optic chiasm.
TS17: oral region	Present	Regionally restricted	1G	Expression is at the buccal roof.

Structure	Level	Pattern	Image	Note
TS17: nasal pit	Present	Regionally restricted	1H	Expression is in the anterior rim of the nasal placode.

Structure	Level	Pattern	Image	Note
TS17: optic cup inner layer	Present	Graded	1I	Expression is most intense in the center.
TS17: lens pit	Absent		1I

Structure	Level	Pattern	Image	Note
TS17: optic cup inner layer	Present	Graded	2A	Expression is most intense in the center.
TS17: lens pit	Absent		2A

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	2B	Expression is in the ventral forebrain underlying buccal ectoderm.
TS17: Rathke's pouch	Present		2B

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	2C	Expression is in the ventro-caudal portion of the early pallium.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	2D	Expression is in the ventral forebrain underlying buccal ectoderm.
TS17: Rathke's pouch	Present		2D

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present	Regionally restricted	2E	Expression is in the neural retina near the pigment epithelium.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	2F	Expression is in cells in the vitreal face of the inner nuclear layer.
TS28: retina ganglion cell layer	Present		2F

Structure	Level	Pattern	Image	Note
TS27: retina	Absent