Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54552
Kim J, et al., Zim1, a maternally expressed mouse Kruppel-type zinc-finger gene located in proximal chromosome 7. Hum Mol Genet. 1999 May;8(5):847-54
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:5316426
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Gene symbol:
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Zim1
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Gene name:
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zinc finger, imprinted 1
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Probe:
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Zim1-pA, Zim1-pB
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Assay notes:
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Imprinted gene assay. The maternal C3Hf allele is distinguished from the M.Spretus paternal allele by a single base change that results in a recognition site for the restriction enzyme MspI in the C3Hf sequence.
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Results
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Image: 3A
| Mol. Wt. Marker Lane |
| Control |
| Control |
| Control |
| P newborn
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TS27: brain
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Present
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Present
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50 µg; total RNA |
(C3Hf x M. spretus)F1 |
| Not Specified |
| P newborn
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TS27: liver
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Absent
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Present
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50 µg; total RNA |
(C3Hf x M. spretus)F1 |
| Not Specified |
| P newborn
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TS27: lung
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Absent
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Present
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50 µg; total RNA |
(C3Hf x M. spretus)F1 |
| Not Specified |
| P newborn
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TS27: metanephros
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Absent
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Present
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50 µg; total RNA |
(C3Hf x M. spretus)F1 |
| Not Specified |
| P newborn
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TS27: testis
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Absent
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Present
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50 µg; total RNA |
(C3Hf x M. spretus)F1 |
| Male |
| P newborn
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TS27: heart
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Absent
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Present
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50 µg; total RNA |
(C3Hf x M. spretus)F1 |
| Not Specified |
| P newborn
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TS27: spleen
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Absent
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Present
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50 µg; total RNA |
(C3Hf x M. spretus)F1 |
| Not Specified |
Notes:
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Analysis Tools