Probe:	Gadd45a cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Weak		2A

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong		2B

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	2C	Expression is in the tip of the closing neural tube.
TS14: prosencephalon	Present	Regionally restricted	2C	Expression is in the tip of the closing neural tube.
TS14: future hindbrain	Absent		2C
TS14: future midbrain	Absent		2C

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	2D	Expression is in the tip of the closing neural tube.
TS14: prosencephalon	Present	Regionally restricted	2D	Expression is in the tip of the closing neural tube.
TS14: nervous system	Present	Regionally restricted	2D , 2E	Expression is in scattered cells of the placodes of trigeminal- and dorsal root ganglia.
TS14: trigeminal neural crest	Present	Regionally restricted	2D , 2E	Expression is in scattered cells of the placodes of trigeminal- and dorsal root ganglia.
TS14: future hindbrain	Absent		2D
TS14: future midbrain	Absent		2D

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present		2F
TS15: facio-acoustic preganglion complex	Present		2F
TS15: glossopharyngeal preganglion	Present		2F
TS15: trigeminal preganglion	Present		2F
TS15: vagus preganglion	Present		2F
TS15: olfactory placode	Present	Regionally restricted	2F	Expression is in the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present		2G
TS15: facio-acoustic preganglion complex	Present		2G
TS15: glossopharyngeal preganglion	Present		2G
TS15: trigeminal preganglion	Present		2G
TS15: vagus preganglion	Present		2G
TS15: olfactory placode	Present	Regionally restricted	2G	Expression is in the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present		2H
TS15: facio-acoustic preganglion complex	Present		2H
TS15: glossopharyngeal preganglion	Present		2H
TS15: trigeminal preganglion	Present		2H
TS15: vagus preganglion	Present		2H
TS15: olfactory placode	Present	Regionally restricted	2H	Expression is in the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS15: diencephalon lamina terminalis	Present		2I
TS15: facio-acoustic preganglion complex	Present		2I
TS15: glossopharyngeal preganglion	Present		2I
TS15: vagus preganglion	Present		2I
TS15: olfactory placode	Present	Regionally restricted	2I	Expression is in the olfactory epithelium.
TS15: tail neural plate	Present	Regionally restricted	2I	Expression is in the caudal neuropore.

Structure	Level	Pattern	Image	Note
TS15: myotome	Weak		2K

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		2L

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		2M

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	2N	Expression is in the posterior-distal mesenchyme subjacent to the AER with a dorsal and a ventral expression domain separated by an area of lower expression in the center.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	2O	Expression is in the posterior-distal mesenchyme subjacent to the AER with a dorsal and a ventral expression domain separated by an area of lower expression in the center.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	2P	Expression is in the posterior-distal mesenchyme subjacent to the AER with a dorsal and a ventral expression domain separated by an area of lower expression in the center.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	2Q	Expression is in the posterior-distal mesenchyme subjacent to the AER with a dorsal and a ventral expression domain separated by an area of lower expression in the center.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		2R

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Moderate
TS11: embryo mesoderm	Weak

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted		Expression is in the lip of the neural fold.

Structure	Level	Pattern	Image	Note
TS15: diencephalon lamina terminalis	Strong	Regionally restricted		Expression is in the tip.
TS15: tail neural plate	Strong	Regionally restricted		Expression is in the tip of the caudal neuropore.
TS15: dorsal root ganglion	Moderate
TS15: facio-acoustic preganglion complex	Moderate
TS15: glossopharyngeal preganglion	Moderate
TS15: vagus preganglion	Moderate
TS15: somite	Weak

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Strong
TS17: tail neural tube	Strong	Regionally restricted		Expression is in the caudal neuropore.
TS17: acoustic ganglion	Moderate
TS17: facial ganglion	Moderate
TS17: glossopharyngeal ganglion	Moderate
TS17: olfactory epithelium	Moderate
TS17: vagus ganglion	Moderate
TS17: forelimb bud mesenchyme	Moderate	Regionally restricted		Expression is in the posterior-distal mesenchyme.
TS17: hindlimb bud mesenchyme	Moderate	Regionally restricted		Expression is in the posterior-distal mesenchyme.
TS17: telencephalon	Weak
TS17: somite	Weak	Regionally restricted		Caudal somites.