Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(tetO-GCaMP2)12IRyu
Name:	transgene insertion 12I, Ron Yu
MGI ID:	MGI:5301601
Transgene:	Tg(tetO-GCaMP2)12IRyu  Location: unknown
Alliance:	Tg(tetO-GCaMP2)12IRyu page

Strain of Origin:

(CBA x FVB)F1

Transgene Type:    Transgenic (Inducible, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Mutation details: This transgene contains a calcium-sensing molecule, GCaMP2, under the transcriptional control of the tetracycline-responsive promoter element (tetO). GCaMP2 consists of, from 5' to 3', a 13-residue peptide of myosin light chain kinase (M13), a circularly permutated eGFP interrupted at residue 145, calmodulin (CaM), and a polyA recognition sequence. Mutations resulting in increased brightness (D180Y and V93I), thermal stability (V163A, and S175G), and GFP dimerization prevention (A206K) were introduced. (J:157448, J:179273)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:179273 Yu R, Direct Data Submission 2012/01/17. MGI Direct Data Submission. 2012;
All:	7 reference(s)