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Gene Expression Data
Assay Details
Assay
Reference: J:168632 Firtina Z, et al., Unfolded Protein Response (UPR) is activated during normal lens development. Gene Expr Patterns. 2011 Jan-Feb;11(1-2):135-43
Assay type: RT-PCR
MGI Accession ID: MGI:5301005
Gene symbol: Xbp1
Gene name: X-box binding protein 1
Probe: Xbp1-pO, Xbp1-pP
Probe preparation: labelled with SYBR green
Assay notes: Real-time RT-PCR analysis was performed to quantify expression. The relative expression of total and spliced transcripts normalized to b2mg is plotted as bar graphs.
Results
Image: 4B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
E14.5 (s) E14.5 TS22: lens Present (a) 0.3 µg FVB/N Not Specified (s) represents the spliced isoform.
E14.5 (u) E14.5 TS22: lens Present 0.3 µg FVB/N Not Specified (u) represents the unspliced isoform.
E15.5 (s) E15.5 TS23: lens Present 0.3 µg FVB/N Not Specified (s) represents the spliced isoform.
E15.5 (u) E15.5 TS23: lens Present 0.3 µg FVB/N Not Specified (u) represents the unspliced isoform.
E16.5 (s) E16.5 TS24: lens Present 0.3 µg FVB/N Not Specified (s) represents the spliced isoform.
E16.5 (u) E16.5 TS24: lens Present 0.3 µg FVB/N Not Specified (u) represents the unspliced isoform.
E17.5 (s) E17.5 TS25: lens Present 0.3 µg FVB/N Not Specified (s) represents the spliced isoform.
E17.5 (u) E17.5 TS25: lens Present 0.3 µg FVB/N Not Specified (u) represents the unspliced isoform.
E18.5 (s) E18.5 TS26: lens Present 0.3 µg FVB/N Not Specified (s) represents the spliced isoform.
E18.5 (u) E18.5 TS26: lens Present 0.3 µg FVB/N Not Specified (u) represents the unspliced isoform.
P3 (s) P3 TS27: lens Present 0.3 µg FVB/N Not Specified (s) represents the spliced isoform.
P3 (u) P3 TS27: lens Present 0.3 µg FVB/N Not Specified (u) represents the unspliced isoform.
P10 (s) P10 TS28: lens Present 0.3 µg FVB/N Not Specified (s) represents the spliced isoform.
P10 (u) P10 TS28: lens Present 0.3 µg FVB/N Not Specified (u) represents the unspliced isoform.
2.5 mo (s) P m 2.5 TS28: lens Present (b) 0.3 µg FVB/N Not Specified (s) represents the spliced isoform.
2.5 mo (u) P m 2.5 TS28: lens Present 0.3 µg FVB/N Not Specified (u) represents the unspliced isoform.
Notes:
(a) The spliced isoform represents about 17% of total Xbp1 transcripts at E14.5.
(b) The spliced isoform represents about 32% of total Xbp1 transcripts at 2.5 m.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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