Gene Expression Data
Assay Details
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Assay
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Reference:
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J:171625
Griesel G, et al., Generation of knockout mice expressing a GFP-reporter under the control of the Lmx1a locus. Gene Expr Patterns. 2011 Jun-Jul;11(5-6):345-8
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5294447
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Gene symbol:
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Lmx1a
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Gene name:
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LIM homeobox transcription factor 1 alpha
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Reporter:
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GFP
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Antibody:
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anti-GFP (abcam)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
2G:
embryonic day 10.5; Lmx1atm1Aman/Lmx1a +
(more  )
Specimen
2G:
(close  )
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Genetic Background:
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involves: 129/Sv * CD-1
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Mutant Allele(s):
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Lmx1atm1Aman/Lmx1a+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2H:
embryonic day 10.5; Lmx1atm1Aman/Lmx1a +
(more )
Specimen
2H:
(close )
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Genetic Background:
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involves: 129/Sv * CD-1
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Mutant Allele(s):
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Lmx1atm1Aman/Lmx1a+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2H
,
2K
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Expression in the rhombic lip.
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Specimen
2I:
embryonic day 10.5; Lmx1atm1Aman/Lmx1a +
(more )
Specimen
2I:
(close )
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Genetic Background:
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involves: 129/Sv * CD-1
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Mutant Allele(s):
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Lmx1atm1Aman/Lmx1a+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
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2I
,
2L
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Expression in the otic vesicle.
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Specimen
3D/H:
embryonic day 12.5-13.5; Lmx1atm1Aman/Lmx1a +
(more )
Specimen
3D/H:
(close )
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Genetic Background:
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involves: 129/Sv * CD-1
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Mutant Allele(s):
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Lmx1atm1Aman/Lmx1a+
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Age:
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embryonic day 12.5-13.5
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Age Note:
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There are discrepancies between the text and the figure legend as to the age of the embryo.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4C:
embryonic day 13.5; Lmx1atm1Aman/Lmx1a +
(more )
Specimen
4C:
(close )
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Genetic Background:
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involves: 129/Sv * CD-1
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Mutant Allele(s):
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Lmx1atm1Aman/Lmx1a+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon |
Present |
Regionally restricted |
4C
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Expression in the cortical hem.
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Specimen
4F:
embryonic day 13.5; Lmx1atm1Aman/Lmx1a +
(more )
Specimen
4F:
(close )
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Genetic Background:
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involves: 129/Sv * CD-1
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Mutant Allele(s):
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Lmx1atm1Aman/Lmx1a+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools