Slc5a6 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:174767 Tang F, et al., Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One. 2011;6(6):e21208
Assay type:	RT-PCR
MGI Accession ID:	MGI:5289285
Gene symbol:	Slc5a6
Gene name:	solute carrier family 5 (sodium-dependent vitamin transporter), member 6

Probe:

Slc5a6-assay_13/17-FP_first allele, RP

Assay notes:

These results were reported in Table S7. They were obtained using TaqMan real time PCR using individual blastomeres from 4-cell embryos. Two different primer sets were used with each sample in order to examine allele specific expression. The results from two assays analyzing expression of this gene were presented in the raw CT values sheet of Table S7; two primer sets were reported in the assays design sheet. However, the assay ids did not match between the two sheets. Thus, it is possible primer set MGI:5289225 (or another, unreported set) was used to obtain these data. In the Supplementary Materials and Methods, the authors reported that a gene was detected when the real-time PCR reaction had a Ct value of <32. Thus, we have interpreted Ct values of<=31.5 as present and >=32.5 as absent.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
4-cell-E1-B1-first allele	E2.0	TS3: 4-cell stage embryo	Present (a)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 1 from embryo 1.
4-cell-E1-B1-second allele	Lane not used in this assay
4-cell-E1-B2-first allele	E2.0	TS3: 4-cell stage embryo	Present (b)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 2 from embryo 1.
4-cell-E1-B2-second allele	Lane not used in this assay
4-cell-E2-B1-first allele	E2.0	TS3: 4-cell stage embryo	Present (c)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 1 from embryo 2.
4-cell-E2-B1-second allele	Lane not used in this assay
4-cell-E2-B2-first allele	E2.0	TS3: 4-cell stage embryo	Present (d)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 2 from embryo 2.
4-cell-E2-B2-second allele	Lane not used in this assay
4-cell-E3-B1-first allele	E2.0	TS3: 4-cell stage embryo	Present (e)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 1 from embryo 3.
4-cell-E3-B1-second allele	Lane not used in this assay
4-cell-E3-B2-first allele	E2.0	TS3: 4-cell stage embryo	Present (f)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 2 from embryo 3.
4-cell-E3-B2-second allele	Lane not used in this assay

Notes:
(a) The averaged Ct value from 3 technical replicates was 26.88122933.
(b) The averaged Ct value from 3 technical replicates was 26.88808267.
(c) The averaged Ct value from 3 technical replicates was 28.15750667.
(d) The averaged Ct value from 3 technical replicates was 26.99549667.
(e) The averaged Ct value from 3 technical replicates was 20.41310133.
(f) The averaged Ct value from 3 technical replicates was 20.10392733.

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