Summary | Transgene origin | Transgene description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Klhl20Tg(Tyr)2372E-3a3Ove
Name:	kelch-like 20; transgene lentiviral insertion 2372E-3a3, Paul A Overbeek
MGI ID:	MGI:5286696
Synonyms:	OVE#2372E-3a3, OVE2372E-3a3
Gene:	Klhl20  Location: Chr1:160915945-160959078 bp, - strand  Genetic Position: Chr1, 69.75 cM
Alliance:	Klhl20Tg(Tyr)2372E-3a3Ove page

Strain of Origin:

FVB/N

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: The Tyro-IRES-sd-loxP-FUGW lentiviral transgene (LV2187) was designed with a mouse tyrosinase minigene (Tyro) followed by an IRES::3xATG::splice-donor::loxP sequence in the FUGW self-inactivating HIV-based lentiviral vector backbone. The Tyro-IRES-sd-loxP replaced the ubiquitin-c promoter, EGFP, and WPRE sequences originally found in the FUGW lentiviral vector. The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in antisense orientation relative to the FUGW backbone. The Tyro minigene is followed by an internal ribosome entry site (IRES) ending with ATGs in all three reading frames (3xATG), an artificial splice donor sequence, and a loxP site in this construct. In line 2372E-3a3, the lentiviral transgene inserted into last intron (12; NCBI37/mm9; 3'-163,021,329(-)) in sense orientation relative to the disrupted gene. (J:175597)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Klhl20 Mutation:	36 strains or lines available

Original:	J:175597 Overbeek PA, Direct Data Submission for Overbeek Lentiviral Transgenic Lines. MGI Direct Data Submission. 2011;
All:	1 reference(s)