Gene Expression Data
Assay Details
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Assay
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Reference:
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J:31260
Wuenschell CW, et al., Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages. J Histochem Cytochem. 1996 Feb;44(2):113-23
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5286165
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Gene symbol:
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Calca
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Gene name:
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calcitonin/calcitonin-related polypeptide, alpha
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Antibody:
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anti-CGRP
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
not shown:
embryonic day 13.0
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung epithelium |
Present |
Regionally restricted |
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Expression in epithelium of smaller airways; no expression in epithelium of larger airways.
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Specimen
1A:
embryonic day 14.0
(more )
Specimen
1A:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung epithelium |
Present |
Regionally restricted |
1A
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Expression in epithelium of smaller, more peripheral airways; expression tended to be continuous, strongest at luminal edge. Little or no expression in epithelium of larger proximal airways.
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Specimen
1D:
embryonic day 14.0
(more )
Specimen
1D:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung epithelium |
Present |
Regionally restricted |
1D
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Expression confined to epithelium of small airways; maximal level of expression not as high as at E14.
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Specimen
not shown:
embryonic day 15.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lung epithelium |
Present |
Regionally restricted |
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Expression confined to epithelium of small airways; maximal level of expression not as high as at E14.
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Specimen
2A:
embryonic day 16.0
(more )
Specimen
2A:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lung epithelium |
Present |
Regionally restricted |
2A
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Low level of expression in epithelium of small airways. Foci of expression representing clusters of pulmonary neuroendocrine cells in epithelium of large airways.
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Specimen
not shown:
embryonic day 17.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: lung epithelium |
Present |
Regionally restricted |
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Pattern of expression similar to those described for more mature specimens of E16 lung tissue.
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Specimen
3A:
embryonic day 18.0
(more )
Specimen
3A:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
Present |
Regionally restricted |
3A
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Expression restricted to scatter foci occurring most frequently in large proximal airway epithelium, and more rarely in small airways.
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Specimen
3C:
embryonic day 18.0
(more )
Specimen
3C:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
Present |
Regionally restricted |
3C
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Expression in clusters of cells with the characteristic appearance of neuroepithelial bodies (NEBs). Expressing cells at the core of the NEB, with no expression in surrounding cells.
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Specimen
4A:
embryonic day 18.0
(more )
Specimen
4A:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
Present |
Regionally restricted |
4A
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Expression in large NEB in large airway epithelium, and very small NEB in small airway.
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Analysis Tools