Antibody:	anti-K12N
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The authors used two antibodies. One was specific for the amino terminus and the other for the carboxyl terminus. Identical results were obtained with each.

Structure	Level	Pattern	Image	Note
TS23: cornea epithelium	Present	Regionally restricted	1_K12 E15.5	Expression is present in the suprabasal epithelial cells.

Structure	Level	Pattern	Image	Note
TS25: cornea epithelium	Present	Regionally restricted	1_K12 E17.5	Expression is present in the suprabasal epithelial cells. This is the time of peak expression.

Structure	Level	Pattern	Image	Note
TS26: cornea epithelium	Present			Expression levels are beginning to decline in the suprabasal epithelial cells.

Structure	Level	Pattern	Image	Note
TS27: cornea epithelium	Trace		1_K12 P0

Structure	Level	Pattern	Image	Note
TS27: cornea epithelium	Trace

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	1_K12 P4	There is no expression in the corneal basal cells.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Non-Uniform	1_K12 P10	Expression is primarily limited to suprabasal and superficial layers.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Non-Uniform	2_P30	A significant number of basal corneal epithelial cells do not stain, although the number that do stain increases as the animals age.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Non-Uniform	2_P90	A significant number of basal corneal epithelial cells do not stain, although the number that do stain increases as the animals age.