Hs3st2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:103005 Yabe T, et al., Developmental and regional expression of heparan sulfate sulfotransferase genes in the mouse brain. Glycobiology. 2005 Oct;15(10):982-93
Assay type:	RT-PCR
MGI Accession ID:	MGI:4949746
Gene symbol:	Hs3st2
Gene name:	heparan sulfate (glucosamine) 3-O-sulfotransferase 2

Probe:	3-OST-2 primer set2
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Expression level was normalized to that of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript. The primers amplify a 170bp fragment.

Results

Image: 6_3-OST-2

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E16	E16.0 (a)	TS24: cerebral cortex TS24: hippocampus	Present	2 µg; total RNA	BALB/c		Not Specified
P1	P1	TS27: cerebral cortex TS27: hippocampus	Present	2 µg; total RNA	BALB/c		Not Specified
P8	P8	TS28: cerebral cortex TS28: hippocampus	Present	2 µg; total RNA	BALB/c		Not Specified
P14	P14	TS28: cerebral cortex TS28: hippocampus	Present	2 µg; total RNA	BALB/c		Not Specified
P21	P21	TS28: cerebral cortex TS28: hippocampus	Present	2 µg; total RNA	BALB/c		Not Specified
Ad	P adult	TS28: cerebral cortex TS28: hippocampus	Present	2 µg; total RNA	BALB/c		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Quantification indicated that this gene was highly expressed in the cerebrum from the embryonic period to adulthood. Expression level sharply increased from E16-P8 (about three times more than at E16), followed by a slight reduction thereafter.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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