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Atf2tm1(NCOM)Mfgc
Targeted Allele Detail
Summary
Symbol: Atf2tm1(NCOM)Mfgc
Name: activating transcription factor 2; targeted mutation 1, Mammalian Functional Genomics Centre
MGI ID: MGI:4880185
Gene: Atf2  Location: Chr2:73646853-73722983 bp, - strand  Genetic Position: Chr2, 43.86 cM
IMPC: Atf2 gene page
Mutation
origin
Mutant Cell Lines:  N00101P0_C_47W_A5, N00101P0_C_47W_A7, N00101P0_C_47W_B7, N00101P0_C_47W_E6, N00101P0_C_47W_F5, N00101P0_C_47W_F6, N00101P0_C_47W_G5, N00101P0_C_47W_G6
Germline Transmission:  Unknown
Parent Cell Line:  C2 (Tcp) (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: NorCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_NTARU-1
 
Mutation detailsThe insertion of NorCOMM cassette pNTARU created a deletion of size 1047bp between positions 73680708-73681755 of Chromosome 2 (Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an F3 site followed by a universal inverse PCR primer site and a splice acceptor lacZ-neomycin phosphotransferase fusion (beta-geo) sequence. This is followed by a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165964)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Atf2 Mutation:  89 strains or lines available
References
Original:  J:165964 Mammalian Functional Genomics Centre, Alleles produced for the NorCOMM project by the Mammalian Functional Genomics Centre (Mfgc), University of Manitoba. MGI Direct Data Submission. 2010;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory