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Col1a1tm1(tetO-Lin28a)Gqda
Targeted Allele Detail
Summary
Symbol: Col1a1tm1(tetO-Lin28a)Gqda
Name: collagen, type I, alpha 1; targeted mutation 1, George Q Daley
MGI ID: MGI:4849370
Synonyms: Lin28a Tg
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance: Col1a1tm1(tetO-Lin28a)Gqda page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:166568
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
 
Col1a1tm1(tetO-Lin28a)Gqda expresses 1 gene
 
Col1a1tm1(tetO-Lin28a)Gqda expression driven by 1 gene
 
Mutation detailsRMCE on KH2 cells inserted a cassette that contains a splice acceptor, 2 polyadenylation sequence, tetracycline operator, flag-tagged mouse cDNA, and an SV40 polyadenylation signal into the 3'UTR. Leaky ectopic expression in the absence of doxycycline induction was confirmed by quantitative RT-PCR and immunohistochemistry. (J:166568)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  160 strains or lines available
Notes
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*))Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae.
References
Original:  J:166568 Zhu H, et al., Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies. Nat Genet. 2010 Jul;42(7):626-30
All:  11 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory