Probe:	Chx10 cDNA6
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	5B , 5A	Expression limited to anterior region of the optic vesicles, evaginating from diencephalon.
TS15: surface ectoderm	Absent		5B , 5A	No expression in overlying head ectoderm, which gives rise to the lens.

Structure	Level	Pattern	Image	Note
TS15: optic stalk	Absent		5C , 5D	No expression in posterior optic vesicle in cells that will form the retinal pigment epithelium.
TS15: optic vesicle	Present	Regionally restricted	5C , 5D
TS15: surface ectoderm	Absent		5D , 5C	No expression in overlying head ectoderm, which gives rise to the lens.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Strong	Uniform	5F , 5E
TS19: pigmented retinal epithelium	Absent		5E , 5F
TS19: eye	Present	Regionally restricted	5E , 5F	Authors state there is no expression in other parts of the eye.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	5H , 5G	Expression restricted to neuroblast layer. No expression in ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS24: retina	Strong	Regionally restricted	5J , 5I	Stronger expression in outer layer of neuroblast cells. No expression in ganglion cells.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		5L , 5K
TS28: retina outer nuclear layer	Absent		5K , 5L

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		5N , 5M
TS28: photoreceptor layer	Absent		5N , 5M
TS28: retina ganglion cell layer	Absent		5N , 5M
TS28: retina outer nuclear layer	Absent		5N , 5M

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted		No expression in cells closest to the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Absent

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present

Structure	Level	Pattern	Image	Note
TS18: metencephalon	Present	Regionally restricted	6A , 6B	Expression extends ventrally throughout posterior part of neural tube, from rhombencephalic isthmus into metencephalon, myelencephalon and spinal cord.
TS18: midbrain-hindbrain junction	Present	Regionally restricted	6A , 6B	Expression extends ventrally throughout posterior part of neural tube, from rhombencephalic isthmus into metencephalon, myelencephalon and spinal cord.
TS18: myelencephalon	Present	Regionally restricted	6A , 6B	Expression extends ventrally throughout posterior part of neural tube, from rhombencephalic isthmus into metencephalon, myelencephalon and spinal cord.
TS18: cerebellum primordium	Absent		6B , 6A

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	6C , 6D	Spatially restricted expression maintained in rhombencephalon during formation of pons and medulla.
TS21: medulla oblongata	Present	Regionally restricted	6D , 6C	Expression localized to medial ventral part of pons, medial ventral medulla oblongata and ventral part of spinal cord.
TS21: pons	Present	Regionally restricted	6D , 6C	Expression localized to medial ventral part of pons, medial ventral medulla oblongata and ventral part of spinal cord.
TS21: spinal cord	Present	Regionally restricted	6D , 6C	Expression localized to medial ventral part of pons, medial ventral medulla oblongata and ventral part of spinal cord.

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Present	Regionally restricted	6F , 6E	Region of expression overlaps with region in which cranial motor nerve nuclei develop, located below the mesencephalic vesicle and fourth ventricle at level of trigeminal, abducens and facial motor nuclei.
TS23: pons	Present	Regionally restricted	6F , 6E	At level of cranial nerves VI and VII, expression below the mesencephalic vesicle, covering regions of abducens and facial motor nuclei in the pons. Expression in regions of medial pons in which some of the reticular nuclei arise.

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Present	Regionally restricted		Authors report a similar pattern of expression in the trigeminal motor nuclei at the level of cranial nerve V.

Structure	Level	Pattern	Image	Note
TS23: medulla oblongata	Present	Regionally restricted	6G , 6H	Expression in the region of the medullary reticular formtion of the medulla oblongata.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	6I , 6J	Expression localized to subset of cells in ventral region of ependymal layer. Minor expression in mantle layer. No Expression in most ventral part of ependymal layer, including floor plate, or in cells just ventral to sulcus limitans.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	6L , 6K	At the cervical level, expression extends out to the dorsal basal plate.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted	6M , 6N	Expression in a subset of postmitotic neurons in the dorsal portion of the ventral spinal cord.
TS23: ventral grey horn	Weak	Regionally restricted	6M , 6N	Restricted expression in the basal plate, only in the dorsal portion of the ventral horn. No expression in the ventral portion of the ventral horn.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Weak	Regionally restricted	6O , 6P	Much weaker expression than at earlier stages.
TS24: ventral grey horn	Present	Regionally restricted	6P , 6O	Expression localized to a subset of neurons in the ventral horn, possibly dorsal medial motor neurons and/or intermediate zone cells.
TS24: intermediate grey horn	Ambiguous		6O , 6P	Expression localized to a subset of neurons in the ventral horn, possibly dorsal medial motor neurons and/or intermediate zone cells.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted		Beginning at E12.5, expression at the border of the caudal thalamus and pretectal area of the rostral midbrain, possibly in the epithalamus.

Structure	Level	Pattern	Image	Note
TS21: head	Present	Regionally restricted	6R , 6Q	Expression at the border of the developing caudal thalamus and the pretectal region of the rostral midbrain, in the pons, and in the medulla oblongata.
TS21: epithalamus	Ambiguous		6R , 6Q	Expression at the border of the developing caudal thalamus and the pretectal region of the rostral midbrain, in the pons, and in the medulla oblongata.

Structure	Level	Pattern	Image	Note
TS23: thalamus	Present	Regionally restricted	6T , 6S	Expression in the region of the dorsal caudal thalamus.

Structure	Level	Pattern	Image	Note
TS21: lip	Present	Regionally restricted		Expression localized to ectoderm of lips, initally at E13.5.

Structure	Level	Pattern	Image	Note
TS22: lower lip	Present	Regionally restricted	6V , 6U	Expression in upper and lower lip, localizing to ectoderm.
TS22: upper lip	Present	Regionally restricted	6V , 6U	Expression in upper and lower lip, localizing to ectoderm.

Structure	Level	Pattern	Image	Note
TS23: upper lip	Present	Regionally restricted	6X , 6W	Expression localized lateral to median groove (philtrum).