Dnm1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:163311 Boumil RM, et al., A missense mutation in a highly conserved alternate exon of dynamin-1 causes epilepsy in fitful mice. PLoS Genet. 2010 Aug;6(8)
Assay type:	RT-PCR
MGI Accession ID:	MGI:4847922
Gene symbol:	Dnm1
Gene name:	dynamin 1

Probe:	ex9F, ex12R
Assay notes:	The PCR products were digested with the HphI restriction enzyme prior to gel electrophoresis to distinguish between the two different isoforms. Quantification of expression is shown in Figure 4B.

Results

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	237 bp (b)	128 bp (d)	109 bp (d)	Amount	Genetic Background	Mutant Allele(s)	Sex
E17.5 +/+	E17.5 (a)	TS25: brain	Present (c)	Present	Present	2 µg; total RNA	C57BL/6J		Not Specified
E17.5 ftfl/ftfl	E17.5 (a)	TS25: brain	Present (e)	Present	Present	2 µg; total RNA	C57BL/6J-Dnm1^Ftfl	Dnm1^Ftfl/Dnm1^Ftfl	Not Specified
P0 +/+	P0	TS27: brain	Present (c)	Present	Present	2 µg; total RNA	C57BL/6J		Not Specified
P0 ftfl/ftfl	P0	TS27: brain	Present (e)	Present	Present	2 µg; total RNA	C57BL/6J-Dnm1^Ftfl	Dnm1^Ftfl/Dnm1^Ftfl	Not Specified
P14 +/+	P14	TS28: brain	Present (c)	Present	Present	2 µg; total RNA	C57BL/6J		Not Specified
P14 ftfl/ftfl	P14	TS28: brain	Present (e)	Present	Present	2 µg; total RNA	C57BL/6J-Dnm1^Ftfl	Dnm1^Ftfl/Dnm1^Ftfl	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Corresponds to the undigested Dnm1a transcript.
(c) The Dnm1a transcript becomes increasingly upregulated during development.
(d) This is one of the two bands representing the digested Dnm1b transcript.
(e) There is a delay in upregulation of the Dnm1a transcript during development in the mutants.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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