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Gene Expression Data
Assay Details
Assay
Reference: J:163311 Boumil RM, et al., A missense mutation in a highly conserved alternate exon of dynamin-1 causes epilepsy in fitful mice. PLoS Genet. 2010 Aug;6(8)
Assay type: RT-PCR
MGI Accession ID: MGI:4847922
Gene symbol: Dnm1
Gene name: dynamin 1
Probe: ex9F, ex12R
Assay notes: The PCR products were digested with the HphI restriction enzyme prior to gel electrophoresis to distinguish between the two different isoforms. Quantification of expression is shown in Figure 4B.
Results
Image: 4A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 237 bp (b) 128 bp (d) 109 bp (d) Amount Genetic BackgroundMutant Allele(s)Sex
E17.5 +/+ E17.5 (a) TS25: brain Present (c) Present Present 2 µg; total RNA C57BL/6J Not Specified
E17.5 ftfl/ftfl E17.5 (a) TS25: brain Present (e) Present Present 2 µg; total RNA C57BL/6J-Dnm1Ftfl Dnm1Ftfl/Dnm1FtflNot Specified
P0 +/+ P0 TS27: brain Present (c) Present Present 2 µg; total RNA C57BL/6J Not Specified
P0 ftfl/ftfl P0 TS27: brain Present (e) Present Present 2 µg; total RNA C57BL/6J-Dnm1Ftfl Dnm1Ftfl/Dnm1FtflNot Specified
P14 +/+ P14 TS28: brain Present (c) Present Present 2 µg; total RNA C57BL/6J Not Specified
P14 ftfl/ftfl P14 TS28: brain Present (e) Present Present 2 µg; total RNA C57BL/6J-Dnm1Ftfl Dnm1Ftfl/Dnm1FtflNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Corresponds to the undigested Dnm1a transcript.
(c) The Dnm1a transcript becomes increasingly upregulated during development.
(d) This is one of the two bands representing the digested Dnm1b transcript.
(e) There is a delay in upregulation of the Dnm1a transcript during development in the mutants.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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