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Gene Expression Data
Assay Details
Assay
Reference: J:160941 Zhang N, et al., The asparaginyl hydroxylase factor inhibiting HIF-1alpha is an essential regulator of metabolism. Cell Metab. 2010 May 5;11(5):364-78
Assay type: Western blot
MGI Accession ID: MGI:4836520
Gene symbol: Prkaa1
Gene name: protein kinase, AMP-activated, alpha 1 catalytic subunit
Antibody: anti-phospho AMPKalpha (#2531)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: This antibody recognizes both Prkaa1 and Prkaa2 when phosphorylated at threonine 172.
Results Image: 3C Phospho-AMPK
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex
FIH KO E12.5 (a) TS20: embryo Present (b) B6.129-Hif1antm1.2Rsjo Hif1antm1.2Rsjo/Hif1antm1.2RsjoNot Specified
FIH KO E12.5 (a) TS20: embryo Present (b) B6.129-Hif1antm1.2Rsjo Hif1antm1.2Rsjo/Hif1antm1.2RsjoNot Specified
FIH KO E12.5 (a) TS20: embryo Present (b) B6.129-Hif1antm1.2Rsjo Hif1antm1.2Rsjo/Hif1antm1.2RsjoNot Specified
FIH KO E12.5 (a) TS20: embryo Present (b) B6.129-Hif1antm1.2Rsjo Hif1antm1.2Rsjo/Hif1antm1.2RsjoNot Specified
FIH KO E12.5 (a) TS20: embryo Present (b) B6.129-Hif1antm1.2Rsjo Hif1antm1.2Rsjo/Hif1antm1.2RsjoNot Specified
WT E12.5 (a) TS20: embryo Present B6.129-Hif1antm1.2Rsjo Not Specified
WT E12.5 (a) TS20: embryo Present B6.129-Hif1antm1.2Rsjo Not Specified
WT E12.5 (a) TS20: embryo Present B6.129-Hif1antm1.2Rsjo Not Specified
FIH Het E12.5 (a) TS20: embryo Present B6.129-Hif1antm1.2Rsjo Hif1antm1.2Rsjo/Hif1an+Not Specified
FIH Het E12.5 (a) TS20: embryo Present B6.129-Hif1antm1.2Rsjo Hif1antm1.2Rsjo/Hif1an+Not Specified
FIH Het E12.5 (a) TS20: embryo Present B6.129-Hif1antm1.2Rsjo Hif1antm1.2Rsjo/Hif1an+Not Specified
FIH Het E12.5 (a) TS20: embryo Present B6.129-Hif1antm1.2Rsjo Hif1antm1.2Rsjo/Hif1an+Not Specified
FIH Het E12.5 (a) TS20: embryo Present B6.129-Hif1antm1.2Rsjo Hif1antm1.2Rsjo/Hif1an+Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Null embryos had suppressed AMPK activation.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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