Tg(Per1-Venus)33Obr
Transgene Detail
|
|
| Symbol: |
Tg(Per1-Venus)33Obr |
| Name: |
transgene insertion 33, Karl Obrietan |
| MGI ID: |
MGI:4456471 |
| Synonyms: |
mPer1-Venus, mPer1-Venus founder line no. 33 |
| Transgene: |
Tg(Per1-Venus)33Obr Location: unknown
|
| Alliance: |
Tg(Per1-Venus)33Obr page
|
|
|
|
| Transgene Type: |
|
Transgenic (Reporter) |
| Mutation: |
|
Insertion
|
| |
|
Mutation details: The transgene comprises bacterial artificial chromosome (BAC) RP24-277K16 containing the mouse period 1 (Per1) gene into which a Venus-NLS-PEST fusion gene has been inserted. Venus is a yellow fluorescent protein enhanced by the introduction of five point mutations. To improve the protein's kinetics, the PEST domain of the mouse ornithine decarboxylase gene was introduced following the penultimate codon of the gene. A nuclear localization signal (NLS) was cloned in-frame between the Venus coding sequence and the PEST domain. The sequence encoding the fusion protein is preceded by a human cytomegalovirus (hCMV) promoter and followed by an SV40 polyadenylation signal. Its robust expression in a population of cells in the suprachiasmatic nuclei (SCN) of the hypothalamus is cyclic and light-inducible; it is also widely expressed in neuronal and non-neuronal cells of brain regions outside the SCN.
(J:150729)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
|
|
The data reported were obtained using transgenic line 33 and were "consistent with results obtained from the other three transgenic lines," 7, 13, 33 and 4126. ( J:150729) |
| Original: |
J:150729 Cheng HY, et al., Segregation of expression of mPeriod gene homologs in neurons and glia: possible divergent roles of mPeriod1 and mPeriod2 in the brain. Hum Mol Genet. 2009 Aug 15;18(16):3110-24 |
| All: |
7 reference(s) |
|
Analysis Tools
