Probe:	UI-M-BH0-air-a-08-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	2a	Expression is present at the anterior border of the prethalamus. It is also present in the dorsal diencephalic border of the eminentia thalami.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	2b	Expression is present at the anterior border of the prethalamus. It is also present in the dorsal diencephalic border of the eminentia thalami.

Structure	Level	Pattern	Image	Note
TS23: thalamus	Present	Regionally restricted	2e	Expression is present at the anterior border of the prethalamus.

Structure	Level	Pattern	Image	Note
TS23: thalamus	Present	Regionally restricted	2f	Expression is present at the anterior border of the prethalamus.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	S3_a	Expression is present at the anterior border of the prethalamus. It is not present in the cortical hem.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	S3_b	Expression is present at the anterior border of the prethalamus. It is not present in the cortical hem.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	S3_c	Expression is present at the anterior border of the prethalamus. It is not present in the cortical hem.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	S3_d , S3_d'	Expression is present at the anterior border of the prethalamus. It is not present in the cortical hem.

Structure	Level	Pattern	Image	Note
TS23: thalamus	Present	Regionally restricted	S3_e	Expression is present at the anterior border of the prethalamus. It is not present in the cortical hem.

Structure	Level	Pattern	Image	Note
TS23: thalamus	Present	Regionally restricted	S3_f	Expression is present at the anterior border of the prethalamus. It is not present in the cortical hem.

Structure	Level	Pattern	Image	Note
TS23: thalamus	Present	Regionally restricted	S3_g	Expression is present at the anterior border of the prethalamus. It is not present in the cortical hem.

Structure	Level	Pattern	Image	Note
TS23: thalamus	Present	Regionally restricted	S3_h	Expression is present at the anterior border of the prethalamus. It is not present in the cortical hem.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Ambiguous		Gdf10-Shh E11.5-coronal , Gdf10-Shh E11.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer	Strong	Non-Uniform	Gdf10-Shh E11.5-coronal , Gdf10-Shh E11.5-sagittal	Staining is strong in the VZ of the prethalamus & weak in PVH nucleus & AVH. Staining is either absent or ubiquitous in the VZ of dorsal thalamus, dorsal & ventral POAs, VMH & ARH nuclei, AVH, anterior & posterior IDs, premam, mam & supramam zones & TT.
TS19: telencephalon mantle layer	Ambiguous		Gdf10-Shh E11.5-sagittal , Gdf10-Shh E11.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Weak	Non-Uniform	Gdf10-Shh E11.5-sagittal , Gdf10-Shh E11.5-coronal	There is weak staining in the ventricular zone of the cerebral cortex. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Gdf10-Shh E12.5-coronal , Gdf10-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Strong		Gdf10-Shh E12.5-coronal , Gdf10-Shh E12.5-sagittal	There is strong staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Gdf10-Shh E12.5-sagittal , Gdf10-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Ambiguous		Gdf10-Shh E12.5-coronal , Gdf10-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer	Ambiguous		Gdf10-Shh E12.5-coronal , Gdf10-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence	Ambiguous		Gdf10-Shh E12.5-sagittal , Gdf10-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer	Weak	Non-Uniform	Gdf10-Shh E12.5-coronal , Gdf10-Shh E12.5-sagittal	There is weak staining in the mantle zone of the AVH. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Weak	Non-Uniform	Gdf10-Shh E12.5-sagittal , Gdf10-Shh E12.5-coronal	There is weak staining in the ventricular zone of the PVH nucleus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer	Ambiguous		Gdf10-Shh E12.5-sagittal , Gdf10-Shh E12.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Strong	Non-Uniform	Gdf10-Shh E12.5-coronal , Gdf10-Shh E12.5-sagittal	There is strong staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Gdf10-Shh E14.5-coronal , Gdf10-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Gdf10-Shh E14.5-sagittal , Gdf10-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Gdf10-Shh E14.5-coronal , Gdf10-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		Gdf10-Shh E14.5-coronal , Gdf10-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Ambiguous		Gdf10-Shh E14.5-coronal , Gdf10-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Ambiguous		Gdf10-Shh E14.5-coronal , Gdf10-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Ambiguous		Gdf10-Shh E14.5-sagittal , Gdf10-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Weak	Non-Uniform	Gdf10-Shh E14.5-coronal , Gdf10-Shh E14.5-sagittal	There is weak staining in the ventricular zone of the PVH nucleus and the AVH. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, VMH and ARH nuclei and anterior and posterior IDs.
TS22: thalamus mantle layer	Ambiguous		Gdf10-Shh E14.5-sagittal , Gdf10-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Strong	Non-Uniform	Gdf10-Shh E14.5-coronal , Gdf10-Shh E14.5-sagittal	There is strong staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Gdf10-Shh E16.5-sagittal , Gdf10-Shh E16.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		Gdf10-Shh E16.5-sagittal , Gdf10-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Gdf10-Shh E16.5-sagittal , Gdf10-Shh E16.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		Gdf10-Shh E16.5-sagittal , Gdf10-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer	Ambiguous		Gdf10-Shh E16.5-sagittal , Gdf10-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence	Ambiguous		Gdf10-Shh E16.5-sagittal , Gdf10-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Ambiguous		Gdf10-Shh E16.5-sagittal , Gdf10-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer	Weak	Non-Uniform	Gdf10-Shh E16.5-coronal , Gdf10-Shh E16.5-sagittal	There is weak staining in the ventricular zone of the PVH nucleus and the DAH. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the SCH, DMH, VMH and ARH nuclei, the AVH and the PH.
TS24: thalamus mantle layer	Ambiguous		Gdf10-Shh E16.5-coronal , Gdf10-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Strong	Non-Uniform	Gdf10-Shh E16.5-coronal , Gdf10-Shh E16.5-sagittal	There is strong staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.