Probe:	UI-M-CG0p-bdf-d-07-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S9_j	Expression is present in progenitor cells in the prethalamus and hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	S9_k	Expression is present in progenitor cells in the prethalamus.
TS20: hypothalamus	Present	Regionally restricted	S9_k	Expression is present in progenitor cells.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	S9_l	Expression is present in progenitor cells.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	S9_m	Expression is present in progenitors cells.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Ambiguous		Igf2bp1-Shh E11.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer	Very strong	Non-Uniform	Igf2bp1-Shh E11.5-coronal	Staining is very strong in the VZ of dorsal thalamus, prethalamus, dorsal & ventral POAs, AVH, anterior & posterior IDs, premam, mam & supramam zones & TT & moderate in VMH nucleus. Staining is either absent or ubiquitous in the VZ of PVH & ARH nuclei.
TS19: telencephalon mantle layer	Ambiguous		Igf2bp1-Shh E11.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Very strong		Igf2bp1-Shh E11.5-coronal	There is very strong staining in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Igf2bp1-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Moderate		Igf2bp1-Shh E12.5-coronal	There is moderate staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Igf2bp1-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Strong	Non-Uniform	Igf2bp1-Shh E12.5-coronal	There is strong staining in the mantle zone of the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and mam zones and the TT.
TS20: diencephalon ventricular layer	Strong	Non-Uniform	Igf2bp1-Shh E12.5-coronal	There is strong staining in the ventricular zone of the premam and mam zones and the TT. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS20: ganglionic eminence	Moderate	Non-Uniform	Igf2bp1-Shh E12.5-coronal	There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Ambiguous		Igf2bp1-Shh E12.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Strong	Non-Uniform	Igf2bp1-Shh E12.5-coronal	There is strong staining in the ventricular zone of the anterior and posterior ID and moderate staining in the dorsal and ventral POAs and the AVH. Staining is either absent or ubiquitous in the ventricular zone of the PVH, VMH and ARH nuclei.
TS20: thalamus mantle layer	Ambiguous		Igf2bp1-Shh E12.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Moderate		Igf2bp1-Shh E12.5-coronal	There is moderate staining in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Moderate	Scattered	Igf2bp1-Shh E14.5-coronal	There is moderate, scattered staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Strong		Igf2bp1-Shh E14.5-coronal	There is strong staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Moderate	Scattered	Igf2bp1-Shh E14.5-coronal	There is moderate, scattered staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		Igf2bp1-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Ambiguous		Igf2bp1-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Moderate	Non-Uniform	Igf2bp1-Shh E14.5-coronal	There is moderate staining in the ventricular zone of the ganglionic eminences and weak, scattered staining in the mantle zone of the ganglionic eminences.
TS22: hypothalamus mantle layer	Ambiguous		Igf2bp1-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Weak	Non-Uniform	Igf2bp1-Shh E14.5-coronal	There is weak staining in the ventricular zone of the ventral POA. Staining is either absent or ubiquitous in the ventricular zone of the dorsal POAs the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer	Weak	Non-Uniform	Igf2bp1-Shh E14.5-coronal	There is weak staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS22: thalamus ventricular layer	Ambiguous		Igf2bp1-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Moderate	Scattered	Igf2bp1-Shh E16.5-coronal	There is moderate, scattered staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Strong		Igf2bp1-Shh E16.5-coronal	There is strong staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Moderate	Scattered	Igf2bp1-Shh E16.5-coronal	There is moderate, scattered staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		Igf2bp1-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer	Weak	Non-Uniform	Igf2bp1-Shh E16.5-coronal	There is weak staining in the ventricular zone of the premam and mam zones. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS24: ganglionic eminence	Weak	Non-Uniform	Igf2bp1-Shh E16.5-coronal	There is weak, scattered staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS24: hypothalamus mantle layer	Ambiguous		Igf2bp1-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer	Weak	Non-Uniform	Igf2bp1-Shh E16.5-coronal	There is weak staining in the ventricular zone of the ARH nucleus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH and VMH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Weak	Non-Uniform	Igf2bp1-Shh E16.5-coronal	There is weak staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS24: thalamus ventricular layer	Ambiguous		Igf2bp1-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.