Probe:	UI-M-BH1-aky-a-04-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Very strong	Non-Uniform	Irs4-Shh E11.5-coronal	There is very strong staining in the MZ of the prethalamus, dorsal & ventral POAs, PVH nucleus, AVH & TT. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus, anterior & posterior IDs, VMH & ARH nuclei & premam, mam & supramam zones.
TS19: diencephalon lateral wall ventricular layer	Very strong	Non-Uniform	Irs4-Shh E11.5-coronal	There is very strong staining in the VZ of the prethalamus, PVH nucleus & AVH. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, dorsal & ventral POAs, VMH & ARH nuclei, anterior & posterior IDs, premam, mam & supramam zones & TT.
TS19: telencephalon mantle layer	Ambiguous		Irs4-Shh E11.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Ambiguous		Irs4-Shh E11.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Irs4-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Irs4-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Irs4-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Very strong	Non-Uniform	Irs4-Shh E12.5-coronal	There is very strong staining in the mantle zone of the TT. Staining is either absent or ubiquitous in the mantle zone of the premam zone.
TS20: diencephalon ventricular layer	Ambiguous		Irs4-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam and mam zones and the TT.
TS20: ganglionic eminence	Ambiguous		Irs4-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer	Strong	Non-Uniform	Irs4-Shh E12.5-coronal	There is strong staining in the mantle zone of the dorsal and ventral POAs. Staining is either absent or ubiquitous in the mantle zone of the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Very strong	Non-Uniform	Irs4-Shh E12.5-coronal	There is very strong staining in the ventricular zone of the dorsal POA, PVH nucleus and the AVH and weak staining in the posterior ID. Staining is either absent or ubiquitous in the ventricular zone of the ventral POA, anterior ID and VMH and ARH nuclei.
TS20: thalamus mantle layer	Ambiguous		Irs4-Shh E12.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Ambiguous		Irs4-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.