Gene Expression Data
Assay Details
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Assay
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Reference:
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J:21265
Dolle P, et al., Coordinate expression of the murine Hox-5 complex homoeobox-containing genes during limb pattern formation. Nature. 1989 Dec 14;342(6251):767-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4442510
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Gene symbol:
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Hoxd4
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Gene name:
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homeobox D4
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Probe:
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Hox-5.1 probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
not shown:
embryonic day 8.75
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1a left:
embryonic day 9.25
(more )
Specimen
1a left:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.25
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: forelimb bud mesenchyme |
Present |
Widespread |
1a left Hox-5.1
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Expression throughout the entire forelimb bud mesoderm; no proximal boundary of expression.
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| TS14: neural tube |
Present |
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1a left Hox-5.1
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Expression in the adjacent axial mesoderm and neural tube
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| TS14: trunk mesenchyme |
Present |
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1a left Hox-5.1
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Expression in the adjacent axial mesoderm and neural tube
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Specimen
1a right:
embryonic day 9.25
(more )
Specimen
1a right:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.25
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: forelimb bud mesenchyme |
Present |
Widespread |
1a right Hox-5.1
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Expression throughout the entire forelimb bud mesoderm.
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| TS14: neural tube |
Present |
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1a right Hox-5.1
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Expression in the adjacent axial mesoderm and neural tube
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| TS14: trunk mesenchyme |
Present |
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1a right Hox-5.1
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Expression in the adjacent axial mesoderm and neural tube
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Specimen
1b:
embryonic day 9.75
(more )
Specimen
1b:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud mesenchyme |
Present |
Widespread |
1b Hox-5.1
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No proximal boundary of expression.
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| TS15: embryo |
Present |
Regionally restricted |
1b Hox-5.1
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Expression in axial tissues.
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Specimen
1c:
embryonic day 10.5
(more )
Specimen
1c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Widespread |
1c Hox-5.1
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No proximal boundary of expression. No proximal boundary of expression.
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| TS17: embryo |
Present |
Scattered |
1c Hox-5.1
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Expression in axial tissues.
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Specimen
1d:
embryonic day 12.5
(more )
Specimen
1d:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb pre-cartilage condensation |
Strong |
Regionally restricted |
1d Hox-5.1
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Expression restricted to central limb precartilage condensations. Strong expression in proximal humeral head blastema.
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Specimen
1e:
embryonic day 12.5
(more )
Specimen
1e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1f:
embryonic day 14.5
(more )
Specimen
1f:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: foot mesenchyme |
Absent |
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1f Hox-5.1
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No expression in mesenchymal cells surrounding the phalangeal cartilage anlage.
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Specimen
not shown:
embryonic day 9.75
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools