Probe:	Ende cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	2A	Expression is present in a small population of epiblast cells in the distal half of the embryo.
TS10: extraembryonic endoderm	Absent		2A

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	2B	Expression is present in a small population of epiblast cells in the distal half of the embryo.
TS10: extraembryonic endoderm	Absent		2B

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	2C	Expression is present in a small population of epiblast cells in the distal half of the embryo.
TS10: extraembryonic endoderm	Absent		2C

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	2D	Expression is present in the distal epiblast.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	2E	Expression is present in the distal epiblast.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	2F	Expression is present in the distal epiblast.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2G	Expression is present in the anterior-most definitive endoderm.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2H	Expression is present in the anterior-most definitive endoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	2I	Expression is present in the midline ectoderm in the distal tip of the embryo.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2J	Expression is present in the anterior-most definitive endoderm; as development progresses this expression then spreads along the proximal half of the embryo to the lateral and posterior definitive endoderm.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2K	Expression is present in the anterior-most definitive endoderm; as development progresses this expression then spreads along the proximal half of the embryo to the lateral and posterior definitive endoderm.
TS11: embryo ectoderm	Present	Regionally restricted	2K	Expression is present in the midline ectoderm in the distal tip of the embryo.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2L	Expression is present in the anterior-most definitive endoderm; as development progresses this expression then spreads along the proximal half of the embryo to the lateral and posterior definitive endoderm.
TS11: embryo ectoderm	Absent		2L
TS11: embryo mesoderm	Absent		2L

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2M	Expression is present in the anterior-most definitive endoderm; as development progresses this expression then spreads along the proximal half of the embryo to the lateral and posterior definitive endoderm.
TS11: embryo ectoderm	Absent		2M
TS11: embryo mesoderm	Absent		2M

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2N	Expression is present in the anterior-most definitive endoderm; as development progresses this expression then spreads along the proximal half of the embryo to the lateral and posterior definitive endoderm.
TS11: embryo ectoderm	Absent		2N
TS11: embryo mesoderm	Absent		2N

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	2O	Expression is present in the midline ectoderm in the distal tip of the embryo.
TS11: embryo mesoderm	Absent		2O

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum	Strong		3A
TS12: hindgut diverticulum	Strong		3A
TS12: embryo ectoderm	Present	Regionally restricted	3A	Expression is present in the distal part of the midline ectoderm and symmetrically expressed in the node region of the embryo. It is absent from the dorsal midline and flanking cells of the developing foregut.

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum	Strong		3B
TS12: hindgut diverticulum	Strong		3B

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum	Present	Regionally restricted	3C inset , 3C	Expression is present in the distal part of the midline ectoderm and symmetrically expressed in the node region of the embryo.

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum	Present		3D , 3H	Expression is enriched in the ventral foregut lip and the ventral part of the foregut invagination.
TS12: embryo ectoderm	Present	Regionally restricted	3D , 3H	Expression is present in the distal part of the midline ectoderm. It is absent from the dorsal midline and flanking cells of the developing foregut.

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum	Present		3I , 3E	Expression is enriched in the ventral foregut lip and the ventral part of the foregut invagination.
TS12: embryo ectoderm	Present	Regionally restricted	3I , 3E	Expression is present in the distal part of the midline ectoderm. It is absent from the dorsal midline and flanking cells of the developing foregut.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present	Regionally restricted	3F	Expression is specific to the ventral aspect of the foregut and absent in the remainder (dorsal and lateral aspects) of the foregut.
TS13: hindgut diverticulum	Present	Widespread	3F

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present	Regionally restricted	3G	Expression is specific to the ventral aspect of the foregut and absent in the remainder (dorsal and lateral aspects) of the foregut.

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum	Present	Widespread	3J

Structure	Level	Pattern	Image	Note
TS14: embryo	Present		3K

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present		3L

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	3M	Expression is present in the posterior region.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		3N
TS15: neural tube	Present	Regionally restricted	3N	Expression is present in the ventral portion of the neural tube in the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	3O	Expression is present in the ventral portion of the neural tube in the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS16: foregut	Present	Regionally restricted	3P	Expression is present in a region overlying the heart and absent elsewhere in the foregut.
TS16: neural tube	Present	Regionally restricted	3P	Expression is present in the ventral portion of the neural tube in the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS16: foregut	Present	Regionally restricted	3Q	Expression is present in a region overlying the heart and absent elsewhere in the foregut.
TS16: neural tube	Present	Regionally restricted	3Q	Expression is present in the ventral portion of the neural tube in the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	3R	Expression is restricted to the ventral half of the neural tube.
TS16: foregut	Present	Regionally restricted	3R	Expression is present in a region overlying the heart and absent elsewhere in the foregut.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	3S	Expression is restricted to the ventral half of the neural tube.
TS16: foregut	Present	Regionally restricted	3S	Expression is present in a region overlying the heart and absent elsewhere in the foregut.

Structure	Level	Pattern	Image	Note
TS17: foregut	Present		3T	Expression levels are reduced from those seen earlier.
TS17: hindgut	Present		3T	Expression levels are reduced from those seen earlier.
TS17: neural tube	Present		3T	Expression levels are reduced from those seen earlier.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present		6E

Structure	Level	Pattern	Image	Note
TS14: foregut-midgut junction	Present	Regionally restricted	6F	Expression is present in the liver bud and foregut-midgut junction but is absent from the pancreatic buds.