Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Col1a1tm1(CAG-Sirt1)Dsin
Name:	collagen, type I, alpha 1; targeted mutation 1, David Sinclair
MGI ID:	MGI:4430570
Synonyms:	Col1a1Sirt1-Tg flox-STOP-flox, ColA1flox-STOP-Sirt1, SIRT1STOP
Gene:	Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1tm1(CAG-Sirt1)Dsin page

Germline Transmission:	Earliest citation of germline transmission: J:134301
Parent Cell Line:	v6.5 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Conditional ready, Inserted expressed sequence) Mutation:    Insertion   Col1a1tm1(CAG-Sirt1)Dsin expresses 1 gene Knock-in expresses: Organism Expressed Gene Note mouse Sirt1 (MGI:2135607)   Mutation details: The SIRT1-Tg flox-STOP-flox construct was designed to insert (from 5' to 3') an FRT site, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a loxP-flanked transcriptional STOP cassette, the mouse sirtuin 1 (Sirt1) cDNA sequence, and a PGK-hygro-polyA sequence (with a second FRT site between the PGK and hygro) into the 3' UTR of the mouse collagen A1 (Col1a1) locus. (J:134301)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	160 strains or lines available

Original:	J:134301 Firestein R, et al., The SIRT1 deacetylase suppresses intestinal tumorigenesis and colon cancer growth. PLoS One. 2008;3(4):e2020
All:	16 reference(s)