Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(tetO-Mrgpra1)1Kdmc
Name:	transgene insertion 1, Ken D McCarthy
MGI ID:	MGI:4429490
Synonyms:	tetO-MrgA1
Transgene:	Tg(tetO-Mrgpra1)1Kdmc  Location: unknown
Alliance:	Tg(tetO-Mrgpra1)1Kdmc page

Strain of Origin:

C57BL/6 x C3H

Transgene Type:    Transgenic (Inducible, Inserted expressed sequence, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Mutation details: The transgene construct encodes mouse MAS-related GPR, member A1 joined at its C-terminus to green fluorescent protein; expression of this fusion protein is regulated by a "tet minimal promoter" comprising seven copies of the tet operator sequence followed by the human cytomegalovirus minimal immediate early (CMV-IE) promoter. The construct also contains, interposed between the promoter and the coding sequence, exon 1, intron 1, and 10 base pairs of exon 2, including the splice acceptor, from the mouse transthyretin gene; and following the coding sequence, the SV40 early region polyadenylation signal. In mice bearing this transgene and expressing a tissue/cell type-specific tetracycline transactivator (tTA) or reverse transactivator (rtTA), expression of GFP-tagged MRGPRA1 is regulated by the presence or absence of doxycycline. (J:126487)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:126487 Fiacco TA, et al., Selective stimulation of astrocyte calcium in situ does not affect neuronal excitatory synaptic activity. Neuron. 2007 May 24;54(4):611-26
All:	11 reference(s)