Gene Expression Data
Assay Details
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Assay
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Reference:
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J:154373
Town L, et al., The metalloendopeptidase gene Pitrm1 is regulated by hedgehog signaling in the developing mouse limb and is expressed in muscle progenitors. Dev Dyn. 2009 Oct 29;238(12):3175-3184
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4417962
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Gene symbol:
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Pitrm1
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Gene name:
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pitrilysin metallepetidase 1
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Probe:
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Pitrm1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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At least three embryos were examined at each stage.
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Results
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Specimen
Not shown:
embryonic day 9.5
(more  )
Specimen
Not shown:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2A:
embryonic day 9.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B:
embryonic day 10.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2C:
embryonic day 11.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not shown:
embryonic day 11.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk mesenchyme |
Present |
Regionally restricted |
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Expression is present in a group of cells located on the torso, just beneath the forelimb.
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Specimen
2D:
embryonic day 12.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: trunk mesenchyme |
Present |
Regionally restricted |
2H
,
2D
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Expression is present in cells located on the torso just beneath the forelimb and on the flank, as well as in the shoulder and neck.
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| TS20: shoulder mesenchyme |
Present |
Regionally restricted |
2D
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Expression is present in cells located on the torso just beneath the forelimb and on the flank, as well as in the shoulder and neck.
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| TS20: vibrissa |
Present |
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2D
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Specimen
2E:
embryonic day 13.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trunk mesenchyme |
Present |
Regionally restricted |
2E
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Expression is present in cells located on the torso that span dorsoventrally throughout the interlimb region.
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Specimen
Not shown:
embryonic day 13.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2F:
embryonic day 14.5
(more )
Specimen
2F:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not shown:
embryonic day 12.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2I:
embryonic day 13.5
(more )
Specimen
2I:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2J:
embryonic day 13.5
(more )
Specimen
2J:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not shown:
embryonic day 14.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2L:
embryonic day 11.5
(more )
Note: This embryo has 46 somites.
Specimen
2L:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This embryo has 46 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
2L
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Expression is present in two migratory streams of cells in the proximal limb; these cells are believed to be muscle cell progenitors.
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Specimen
2N:
embryonic day 11.5
(more )
Specimen
2N:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk dermomyotome |
Present |
Regionally restricted |
2N
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Expression is present in the limb flank region in the lateral dermomyotome where hypaxial muscle progenitors originate.
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Specimen
Not shown:
embryonic day 10.0
(more )
Note: This embryo has 32 somites.
Specimen
Not shown:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This embryo has 32 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: limb |
Absent |
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Expression is not detectable above background levels.
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Specimen
4A:
embryonic day 11.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4B:
embryonic day 12.5
(more )
Specimen
4B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4D:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb mesenchyme |
Present |
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4D
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Unlike in wild type, expression is expanded into the anterior domain.
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Specimen
4E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * MF1
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Mutant Allele(s):
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Ptch1tm1Bjw/Ptch1tm1Bjw
Tg(Prrx1-cre)1Cjt/0
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant.
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Specimen
4F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * MF1
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Mutant Allele(s):
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Ptch1tm1Bjw/Ptch1tm1Bjw
Tg(Prrx1-cre)1Cjt/0
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb mesenchyme |
Present |
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4F
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Unlike in wild type, expression is expanded into the anterior domain.
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Specimen
4G:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Shhtm1(EGFP/cre)Cjt/Shhtm1(EGFP/cre)Cjt
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
Not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Shhtm1(EGFP/cre)Cjt/Shhtm1(EGFP/cre)Cjt
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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