About   Help   FAQ
Ldahtm1(KOMP)Vlcg
Targeted Allele Detail
Summary
Symbol: Ldahtm1(KOMP)Vlcg
Name: lipid droplet associated hydrolase; targeted mutation 1, Velocigene
MGI ID: MGI:4399711
Gene: Ldah  Location: Chr12:8258107-8335759 bp, + strand  Genetic Position: Chr12, 3.85 cM
Alliance: Ldahtm1(KOMP)Vlcg page
IMPC: Ldah gene page
Mutation
origin
Mutant Cell Lines:  14003A-A7, 14003A-D9, 14003A-H3
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  VGB6 (ES Cell)
Strain of Origin:  C57BL/6NTac
Project Collection: KOMP-Regeneron
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: ZEN-Ub1
 
Mutation detailsThe insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 18006bp between positions 8270605-8288610 of Chromosome 12 (Build GRCm39). Sequencing confirmed replacement of a region from exon 2, downstream of the start codon, to base 169 of exon 3, including the predicted catalytic Gx-SxG motif, with a lacZ reporter and a loxP-flanked neomycin cassette. Western blot analysis using an antibody binding a C-terminal antigen revealed no detectable protein in the liver, BAT and WAT, adrenal gland, prostate, brain, spleen, kidney, testis, and bone marrow-derived macrophages of homozygous mutant mice. Mass spectrometry analysis confirmed the absence of encoded peptides in WAT and liver lysates. (J:136110, J:237921)
Generation of the Ldahtm1(KOMP)Vlcg allele
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Ldah Mutation:  23 strains or lines available
References
Original:  J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/19/2024
MGI 6.23
The Jackson Laboratory