Gene Expression Data
Assay Details
Assay
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Reference:
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J:3078
Su H, et al., Demonstration of a stage-specific expression of the ZFY protein in fetal mouse testis using anti-peptide antibodies. Mol Reprod Dev. 1992 Nov;33(3):252-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4366904
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Gene symbol:
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Zfy2
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Gene name:
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zinc finger protein 2, Y-linked
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Antibody:
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MoY1-A antiserum
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Assay notes:
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Fixation was with Bouin-Holland solution. Other antisera to the MoY-1 peptide [MGI:4366900], MoY-2 peptide [MGI:4366901] or MoY-5 peptide [MGI:4366902] may have been used. Authors state that the antisera were equally efficient in obtaining the same immunostaining pattern.
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Results
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Specimen
3A:
embryonic day 12.5
(more )
Specimen
3A:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: testis |
Present |
Regionally restricted |
3A
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Expression mainly localized within the seminiferous tubules.
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Specimen
3B:
embryonic day 12.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Male
|
Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: testis |
Present |
Regionally restricted |
3B
|
Expression mainly localized within the seminiferous tubules.
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: reproductive system |
Present |
Regionally restricted |
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Authors report relatively intense expression around the mesonephric ducts.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: paramesonephric duct |
Present |
Regionally restricted |
|
Authors report relatively intense expression around the paramesonephric ducts.
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Specimen
4A:
embryonic day 12.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
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Female
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
|
Paraffin
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Specimen
4B:
embryonic day 12.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Male
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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