Iftap<tm1a(KOMP)Wtsi> Targeted Allele Detail MGI Mouse (MGI:4362302)

Iftap^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

Symbol:	Iftap^{tm1a(KOMP)Wtsi}
Name:	intraflagellar transport associated protein; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID:	MGI:4362302
Synonyms:	B230118H07Rik^{tm1a(KOMP)Wtsi}, NWC-KOMP
Gene:	Iftap Location: Chr2:101391124-101459376 bp, - strand Genetic Position: Chr2, 53.83 cM, cytoband E2
Alliance:	Iftap^{tm1a(KOMP)Wtsi} page
IMPC:	Iftap gene page

Mutation
origin

Mutant Cell Lines:	EPD0067_1_A05, EPD0067_1_B06, EPD0067_1_C04, EPD0067_1_C06, EPD0067_1_D04, EPD0067_1_D05, EPD0067_1_E04, EPD0067_1_E05, EPD0067_1_G04, EPD0067_1_G06, EPD0067_1_H04
Germline Transmission:	Earliest citation of germline transmission: J:236765
Parent Cell Line:	JM8.F6 (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	KOMP-CSD

Mutation
description

Allele Type:		Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter)
Mutation:		Insertion Vector: L1L2_gt0
		Mutation details: The L1L2_gt0 cassette was inserted at position 101413619 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 101414384. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 2 lines available
Carrying any Iftap Mutation:	21 strains or lines available

Notes

Special note about mutant cell line EPD0067_1_B06 used in J:236765: The mutation generated in this ES cell line results in a hypomorphic allele. RT-PCR analysis revealed that synthesis of full-length transcript is strongly suppressed but not completely abrogated in homozygous mutant testes.

References

Original:	J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:	3 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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