Piezo1<Gt(RST343)Byg> Gene trapped Allele Detail MGI Mouse (MGI:4126380)

Piezo1^{Gt(RST343)Byg}
Gene trapped Allele Detail

Summary

Symbol:	Piezo1^{Gt(RST343)Byg}
Name:	piezo-type mechanosensitive ion channel component 1; gene trap RST343, BayGenomics
MGI ID:	MGI:4126380
Synonyms:	Piezo1^gt
Gene:	Piezo1 Location: Chr8:123208437-123278068 bp, - strand Genetic Position: Chr8, 71.31 cM
Alliance:	Piezo1^{Gt(RST343)Byg} page

Growth retardation and pericardial effusion in Piezo1^{Gt(RST343)Byg}/Piezo1^{Gt(RST343)Byg} embryos

Show the 3 phenotype image(s) involving this allele.

Mutation
origin

Mutant Cell Line:	RST343
Germline Transmission:	Earliest citation of germline transmission: J:212179
Parent Cell Line:	Other (see notes) (ES Cell)
Strain of Origin:	129P2/OlaHsd

Mutation
description

Allele Type:

Gene trapped (Reporter)

Mutation:

Insertion of gene trap vector Vector: pGT0TMpfs Vector Type: secretory trap

Mutation details: A gene trap vector randomly integrated in the intron between exons 18 and 19 of the gene. The gene trap cassette contains a splice acceptor that co-opts the endogenous splicing of the mRNA transcript, leading to an in-frame fusion protein containing the first 823 residues of the encoded protein and beta-galactosidase. This gene trap vector also provides a human placental alkaline phosphatase (PLAP) reporter gene expressed through an internal ribosome entry site (IRES). Quantitative PCR analysis using a probe downstream of the gene trap integration site confirmed loss of wild-type transcript in mutant embryos. (J:212179)

Sequence Tags:

Sequence tag details (1 tag)

Genome Context:

Genome Browser view of this mutation

Generation of the Piezo1^{Gt(RST343)Byg} allele

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	9 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Piezo1 Mutation:	87 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

Original:	J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008;
All:	7 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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