Pparg RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:146993 Wyrwoll CS, et al., Altered placental function of 11beta-hydroxysteroid dehydrogenase 2 knockout mice. Endocrinology. 2009 Mar;150(3):1287-93
Assay type:	RT-PCR
MGI Accession ID:	MGI:3851589
Gene symbol:	Pparg
Gene name:	peroxisome proliferator activated receptor gamma

Probe:	Mm00440945_m1
Assay notes:	Real-time quantitative PCR. Placental zones were separated by blunt dissection and the labyrinth zone was used for analysis.

Results

Image: 4B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
E15 +/+	E14.5 (a)	TS22: extraembryonic component TS28: placenta	Present	1 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J		Not Specified
E15 +/-	E14.5 (a)	TS22: extraembryonic component TS28: placenta	Present	1 µg; total RNA	B6.129P2-Hsd11b2^tm1Yko	Hsd11b2^tm1Yko/Hsd11b2⁺	Not Specified
E15 -/-	E14.5 (a)	TS22: extraembryonic component TS28: placenta	Present (b)	1 µg; total RNA	B6.129P2-Hsd11b2^tm1Yko	Hsd11b2^tm1Yko/Hsd11b2^tm1Yko	Not Specified
E18 +/+	E17.5 (a)	TS25: extraembryonic component TS28: placenta	Strong (c)	1 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J		Not Specified
E18 +/-	E17.5 (a)	TS25: extraembryonic component TS28: placenta	Strong (c)	1 µg; total RNA	B6.129P2-Hsd11b2^tm1Yko	Hsd11b2^tm1Yko/Hsd11b2⁺	Not Specified
E18 -/-	E17.5 (a)	TS25: extraembryonic component TS28: placenta	Present (d)	1 µg; total RNA	B6.129P2-Hsd11b2^tm1Yko	Hsd11b2^tm1Yko/Hsd11b2^tm1Yko	Not Specified

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression is similar as in wild-type.
(c) There was an increase in expression compared to E15.
(d) Expression was about half of that seen in wild-type.

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