Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Cd28tm2.1Jmg
Name:	CD28 antigen; targeted mutation 2.1, Jonathan M Green
MGI ID:	MGI:3850854
Synonyms:	CD28-Y170F
Gene:	Cd28  Location: Chr1:60785547-60812521 bp, + strand  Genetic Position: Chr1, 30.52 cM, cytoband C1-C3
Alliance:	Cd28tm2.1Jmg page

Germline Transmission:	Earliest citation of germline transmission: J:149888
Parent Cell Line:	RW-4 (ES Cell)
Strain of Origin:	129X1/SvJ

Allele Type:    Targeted (Not Specified) Mutation:    Nucleotide substitutions   Mutation details: A TAC to TTC mutation was created in exon 4 to substitute phenylalanine for tyrosine at position 170 (Y170F). A floxed neomycin cassette was inserted upstream of the exon and was subsequently removed by cre-mediated recombination. Expression of the mutant allele in splenocytes and thymocytes was similar to endogenous levels as determined by flow cytometry analysis. This mutation disrupts the motif responsible for interaction with the p85 subunit of PI3-kinase. (J:149888)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cd28 Mutation:	53 strains or lines available

Original:	J:149888 Dodson LF, et al., Targeted knock-in mice expressing mutations of CD28 reveal an essential pathway for costimulation. Mol Cell Biol. 2009 Jul;29(13):3710-21
All:	11 reference(s)