Probe:	CATS probe2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Widespread	2A
TS15: branchial arch	Strong		2A
TS15: olfactory placode	Strong		2A
TS15: heart	Absent		2A

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Widespread	2B
TS15: neural tube	Strong		2B
TS15: olfactory placode	Strong		2B
TS15: somite	Strong		2B

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Strong	Regionally restricted	2D	Prominent expression in the posterior region of the midbrain.
TS15: olfactory placode	Strong		2D

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Widespread	2E	Authors report reduction of widespread expression.
TS17: 1st branchial arch mandibular component	Present		2E
TS17: 1st branchial arch maxillary component	Present		2E
TS17: forelimb bud	Present		2E
TS17: hindbrain	Present		2E
TS17: hindlimb bud	Present		2E
TS17: nasal process	Present		2E
TS17: telencephalon	Present		2E
TS17: eye	Present	Regionally restricted	2E	Authors report expression in the lens vesicle.
TS17: midbrain	Present	Regionally restricted	2E	Expression in anterior and posterior regions of midbrain.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Widespread	2F	Authors report reduction of widespread expression.
TS17: tail neural tube	Present		2F

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Widespread	2G	Authors report reduction of widespread expression.
TS17: hindlimb bud	Present		2G
TS17: neural tube	Present		2G

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Strong		2H

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong		2I

Structure	Level	Pattern	Image	Note
TS19: embryo	Present		2J	Authors report reduction of widespread expression.
TS19: forelimb bud	Strong		2J
TS19: hindlimb bud	Strong		2J

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		2K	Authors report reduction of widespread expression.
TS20: forelimb	Strong		2K
TS20: hindlimb	Strong		2K

Structure	Level	Pattern	Image	Note
TS21: embryo	Present		2L	Authors report reduction of widespread expression.
TS21: forelimb	Strong		2L
TS21: hindlimb	Strong		2L

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		2M
TS19: neural tube	Present		2M

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Weak		2N	Abrupt reduction of expression in the hindbrain and neural tube.
TS20: spinal cord	Weak		2N	Abrupt reduction of expression in the hindbrain and neural tube.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		2O

Structure	Level	Pattern	Image	Note
TS17: eye	Present	Regionally restricted	2P	Authors report expression in the lens vesicle.

Structure	Level	Pattern	Image	Note
TS17: eye	Present	Regionally restricted	2Q	Expression in the lens vesicle.

Structure	Level	Pattern	Image	Note
TS21: genital tubercle	Strong		2R

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present		2S
TS22: vibrissa follicle	Present		2S
TS22: head	Absent		2S
TS22: spinal cord	Absent		2S

Structure	Level	Pattern	Image	Note
TS21: vibrissa follicle	Present		2T

Structure	Level	Pattern	Image	Note
TS21: vibrissa follicle	Present		2U

Structure	Level	Pattern	Image	Note
TS19: eye	Present	Regionally restricted	2V	Expression in the neural layer of optic cup.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	3A upper	Expression more prominent in the distal part of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	3A lower	Expression more prominent in the distal part of the hindlimb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		3B upper

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	3B lower	Expression in the dorsal and ventral mesenchyme.
TS19: footplate apical ectodermal ridge	Absent		3B lower

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	3C upper	Expression in the distal part of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	3C lower	Expression in the distal part of the hindlimb bud.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	3D upper	Expression stronger in the posterior domain than in the anterior domain.

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Present		3D lower
TS20: foot epithelium	Absent		3D lower	No expression in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit	Present		3E upper	Expression confined to the digits.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit	Present		3E lower	Expression confined to the digits.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present		3F upper

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present		3F lower

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present		3G upper	Expression confined to the digits.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit	Present		3G lower	Expression confined to the digits.

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present		3H upper

Structure	Level	Pattern	Image	Note
TS22: hindlimb	Present		3H lower