Cyp11a1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:46691 O'Shaughnessy PJ, et al., Fetal development of Leydig cell activity in the mouse is independent of pituitary gonadotroph function. Endocrinology. 1998 Mar;139(3):1141-6
Assay type:	RT-PCR
MGI Accession ID:	MGI:3845604
Gene symbol:	Cyp11a1
Gene name:	cytochrome P450, family 11, subfamily a, polypeptide 1

Probe:	P450scc-pA, P450scc-pB
Probe preparation:	DNA
Assay notes:	Data not shown from semiquantitative RT-PCR; results presented as bar graphs in Fig. 3B.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
not shown	E13.0	TS21: testis	Present (a)	Not Specified; total RNA	Not Specified		Male
not shown	E14.0	TS22: testis	Present (a)	Not Specified; total RNA	Not Specified		Male
not shown	E16.0	TS24: testis	Present (a)	Not Specified; total RNA	Not Specified		Male
not shown	E18.0	TS26: testis	Present (a)	Not Specified; total RNA	Not Specified		Male
not shown	P1	TS27: testis	Present (b)	Not Specified; total RNA	Not Specified		Male
not shown	P5	TS28: testis	Present (b)	Not Specified; total RNA	Not Specified		Male
not shown	E13.0	TS21: testis	Present (c)	Not Specified; total RNA	involves: 101/H * C3H/HeH	Gnrh1^hpg/Gnrh1^hpg	Male
not shown	E14.0	TS22: testis	Present (c)	Not Specified; total RNA	involves: 101/H * C3H/HeH	Gnrh1^hpg/Gnrh1^hpg	Male
not shown	E16.0	TS24: testis	Present (c)	Not Specified; total RNA	involves: 101/H * C3H/HeH	Gnrh1^hpg/Gnrh1^hpg	Male
not shown	E18.0	TS26: testis	Present (c)	Not Specified; total RNA	involves: 101/H * C3H/HeH	Gnrh1^hpg/Gnrh1^hpg	Male
not shown	P1	TS27: testis	Present (d)	Not Specified; total RNA	involves: 101/H * C3H/HeH	Gnrh1^hpg/Gnrh1^hpg	Male
not shown	P5	TS28: testis	Present (d)	Not Specified; total RNA	involves: 101/H * C3H/HeH	Gnrh1^hpg/Gnrh1^hpg	Male

Notes:
(a) Levels increase from E13 up until day of birth.
(b) Levels decrease from day 1 to day 5.
(c) Levels in hpg mice were normal up to E18.
(d) Levels were significantly reduced compared with normal values.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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