Sparc Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:24183 Howe CC, et al., Expression of SPARC/osteonectin transcript in murine embryos and gonads. Differentiation. 1988;37(1):20-5
Assay type:	Northern blot
MGI Accession ID:	MGI:3845478
Gene symbol:	Sparc
Gene name:	secreted acidic cysteine rich glycoprotein

Probe:	p2-4
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	2.1 kb	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
not shown	E8.5 (a)	TS13: embryo	Absent	Not Specified; total RNA	(C57BL/6 x DBA/2)F1		Not Specified
not shown	E12.5 (a)	TS20: embryo	Present (b)	Not Specified; total RNA	(C57BL/6 x DBA/2)F1		Not Specified
not shown	E18.5 (a)	TS26: embryo	Present (c)	Not Specified; total RNA	(C57BL/6 x DBA/2)F1		Not Specified
not shown	E12.5 (a)	TS20: amnion TS20: yolk sac	Present (d)	Not Specified; total RNA	Not Specified		Not Specified	Amnion and visceral yolk sac.
not shown	E18.5 (a)	TS26: amnion TS26: yolk sac	Present (e)	Not Specified; total RNA	Not Specified		Not Specified	Amnion and visceral yolk sac.

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Transcript became increasingly abundant through day 12.
(c) Levels remained elevated through day 18.
(d) Transcripts reached a plateau by day 12.
(e) Transcripts remained at approximately the same level through day 18.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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