Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:138310
Xu C, et al., Expression of Robo/Slit and Semaphorin/Plexin/Neuropilin family members in the developing hypothalamic paraventricular and supraoptic nuclei. Gene Expr Patterns. 2008 Sep;8(7-8):502-7
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3845312
|
|
Gene symbol:
|
Sema6a
|
|
Gene name:
|
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
|
|
Probe:
|
Sema6a cDNA1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
Fixative was Methancarn (60% methanol, 30% chloroform, and 10% acetic acid). These data are described in the text.
|
|
|
Results
|
Specimen
Not shown E12.5:
embryonic day 12.5
(more  )
Specimen
Not shown E12.5:
(close  )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Absent |
|
|
Expression is absent in the paraventricular nucleus/supraoptic nucleus progenitor region.
|
Specimen
Not shown E13.5:
embryonic day 13.5
(more )
Specimen
Not shown E13.5:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus |
Absent |
|
|
Expression is absent in the paraventricular nucleus/supraoptic nucleus progenitor region.
|
Specimen
Not shown E14.5:
embryonic day 14.5
(more )
Specimen
Not shown E14.5:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Absent |
|
|
Expression is absent in the paraventricular nucleus/supraoptic nucleus progenitor region.
|
Specimen
Not shown E15.5:
embryonic day 15.5
(more )
Specimen
Not shown E15.5:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Absent |
|
|
Expression is absent in the paraventricular nucleus/supraoptic nucleus progenitor region.
|
|
Analysis Tools