Gene Expression Data
Assay Details
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Assay
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Reference:
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J:138310
Xu C, et al., Expression of Robo/Slit and Semaphorin/Plexin/Neuropilin family members in the developing hypothalamic paraventricular and supraoptic nuclei. Gene Expr Patterns. 2008 Sep;8(7-8):502-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3845302
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Gene symbol:
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Sema3f
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Gene name:
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sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
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Probe:
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Sema3f cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Fixative was Methancarn (60% methanol,
30% chloroform, and 10% acetic acid).
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Results
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Specimen
3B1, B5:
embryonic day 12.5
(more  )
Note: (B5) is a higher magnification of the boxed area in (B1).
Specimen
3B1, B5:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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(B5) is a higher magnification of the boxed area in (B1).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Present |
Regionally restricted |
3_B1
,
3_B5
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Expression is in the lateral portion of the paraventricular nucleus/supraoptic nucleus progenitor region.
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Specimen
3B2:
embryonic day 13.5
(more )
Specimen
3B2:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus |
Present |
Regionally restricted |
3_B2
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Expression is in cells ventral and dorsal to the paraventricular nucleus.
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Specimen
3B3:
embryonic day 14.5
(more )
Specimen
3B3:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
3_B3
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Expression is in cells ventral and dorsal to the paraventricular nucleus.
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Specimen
3B4:
embryonic day 15.5
(more )
Specimen
3B4:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Weak |
Regionally restricted |
3_B4
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Expression is in the medial-ventral and lateral paraventricular nucleus and in cells ventral and dorsal to the paraventricular nucleus.
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Analysis Tools