Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol: Rev3ltm1Rsky Name: REV3 like, DNA directed polymerase zeta catalytic subunit; targeted mutation 1, Klaus Rajewsky MGI ID: MGI:3837724 Synonyms: Pol-zetaf Gene: Rev3l  Location: Chr10:39608114-39751207 bp, + strand  Genetic Position: Chr10, 21.04 cM Alliance: Rev3ltm1Rsky page

Chromosome aberrations in B cells of Rev3ltm1Rsky/Rev3ltm1.1Rsky Tg(Cr2-cre)3Cgn/0 mice Show the 1 phenotype image(s) involving this allele.

Germline Transmission:	Earliest citation of germline transmission: J:146453
Parent Cell Line:	Bruce 4 (ES Cell)
Strain of Origin:	B6.Cg-Thy1a

Allele Type:    Targeted (Conditional ready, No functional change) Mutation:    Insertion   Mutation details: Exon 2 was targeted conditional deletion becuase messenger RNA splicing from exon 1 to exon 3 would result in a frameshift mutation. A floxed neomycin resistance cassette was placed upstream of the exon, and an additional loxP site was inserted downstream of the exon. After targeting, the neomycin cassette was removed by transient expression of Cre recombinase. Genomic PCR confirmed correct targeting of the locus. (J:146453)

Generation of the Rev3ltm1Rsky and Rev3ltm1.1Rsky alleles

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rev3l Mutation:	118 strains or lines available

Original:	J:146453 Schenten D, et al., Pol zeta ablation in B cells impairs the germinal center reaction, class switch recombination, DNA break repair, and genome stability. J Exp Med. 2009 Feb 16;206(2):477-90
All:	2 reference(s)