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Gene Expression Data
Assay Details
Assay
Reference: J:80962 Sakagami H, et al., Transient up-regulation of elongation factor-2 kinase (Ca2+/calmodulin-dependent protein kinase III) messenger RNA in developing mouse brain. Neurosci Lett. 2002 Sep 13;330(1):41-4
Assay type: Northern blot
MGI Accession ID: MGI:3836766
Gene symbol: Eef2
Gene name: eukaryotic translation elongation factor 2
Probe: eEF-2 cDNA1
Probe preparation: labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: A graph of values of the hybridization bands normalized to control were shown in Fig. 1C.
Results Image: 1B
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
E13 E13.0 (a) TS21: brain Present 10 µg; total RNA Not Specified Not Specified
E15 E15.0 (a) TS23: brain Present (b) 10 µg; total RNA Not Specified Not Specified
E18 E18.0 (a) TS26: brain Present 10 µg; total RNA Not Specified Not Specified
P5 P5 TS28: brain Present (c) 10 µg; total RNA Not Specified Not Specified
P10 P10 TS28: brain Present (c) 10 µg; total RNA Not Specified Not Specified
P15 P15 TS28: brain Present (c) 10 µg; total RNA Not Specified Not Specified
P20 P20 TS28: brain Present (c) 10 µg; total RNA Not Specified Not Specified
P50 P50 TS28: brain Present (c) 10 µg; total RNA Not Specified Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression level peaked at E15.
(c) Expression was sustained at a substantial level until P5. After P5, the expression decreased by approximately 50%.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory