Ube2l3 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:36254 Harbers K, et al., Provirus integration into a gene encoding a ubiquitin-conjugating enzyme results in a placental defect and embryonic lethality. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12412-7
Assay type:	Northern blot
MGI Accession ID:	MGI:3836411
Gene symbol:	Ube2l3
Gene name:	ubiquitin-conjugating enzyme E2L 3

Probe:	UbcM4
Probe preparation:	Double stranded labelled with P32 DNA

Results

Image: 7 ©

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	2.9 kb	2.6 kb	1.8 kb	0.9 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
A6/A6	E15.5	TS23: embryo	Present (a)	Present (a)	Present (a)	Present (a)	15 µg; total RNA	involves: 129/Sv * 129S4/SvJae	Ube2l3^m1Hrb/Ube2l3^m1Hrb	Not Specified
A6/A6	E15.5	TS23: embryo	Present (a)	Present (a)	Present (a)	Present (a)	15 µg; total RNA	involves: 129/Sv * 129S4/SvJae	Ube2l3^m1Hrb/Ube2l3^m1Hrb	Not Specified
A6/wt	E15.5	TS23: embryo	Present (b)	Present (b)	Present (b)	Present (b)	15 µg; total RNA	involves: 129 * 129S4/SvJae	Ube2l3^m1Hrb/Ube2l3⁺	Not Specified
A6/wt	E15.5	TS23: embryo	Present (b)	Present (b)	Present (b)	Present (b)	15 µg; total RNA	involves: 129 * 129S4/SvJae	Ube2l3^m1Hrb/Ube2l3⁺	Not Specified
wt/wt	E15.5	TS23: embryo	Present	Present	Present	Present	15 µg; total RNA	involves: 129 * 129S4/SvJae		Not Specified
wt/wt	E15.5	TS23: embryo	Present	Present	Present	Present	15 µg; total RNA	involves: 129 * 129S4/SvJae		Not Specified
A6/A6	E15.5	TS28: placenta	Present (a)	Present (a)	Present (a)	Present (a)	15 µg; total RNA	involves: 129/Sv * 129S4/SvJae	Ube2l3^m1Hrb/Ube2l3^m1Hrb	Not Specified
A6/A6	E15.5	TS28: placenta	Present (a)	Present (a)	Present (a)	Present (a)	15 µg; total RNA	involves: 129/Sv * 129S4/SvJae	Ube2l3^m1Hrb/Ube2l3^m1Hrb	Not Specified
A6/wt	E15.5	TS28: placenta	Present (b)	Present (b)	Present (b)	Present (b)	15 µg; total RNA	involves: 129 * 129S4/SvJae	Ube2l3^m1Hrb/Ube2l3⁺	Not Specified
A6/wt	E15.5	TS28: placenta	Present (b)	Present (b)	Present (b)	Present (b)	15 µg; total RNA	involves: 129 * 129S4/SvJae	Ube2l3^m1Hrb/Ube2l3⁺	Not Specified
wt/wt	E15.5	TS28: placenta	Present	Present	Present	Present	15 µg; total RNA	involves: 129 * 129S4/SvJae		Not Specified
wt/wt	E15.5	TS28: placenta	Present	Present	Present	Present	15 µg; total RNA	involves: 129 * 129S4/SvJae		Not Specified

Notes:
(a) Expression levels in the homozygote are reduced 70% compared to those seen in wild type.
(b) Expression levels in the heterozygote are reduced 30% compared to those seen in wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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