Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Kit-EGFP)1Mik
Name:	transgene insertion 1, Michael I Kotlikoff
MGI ID:	MGI:3835314
Synonyms:	c-kitBAC-EGFP, Tg(Kit-EGFP)1Mik
Transgene:	Tg(Kit-EGFP)1Mik  Location: unknown
Alliance:	Tg(Kit-EGFP)1Mik page

Strain of Origin:

(C57BL/6 x DBA/2)F2

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(Kit-EGFP)1Mik expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse Kit (MGI:96677)   Mutation details: The transgene is a bacterial artificial chromosome (BAC), RP24-330G11, bearing a 186 kb mouse genomic segment that contains the Kit oncogene, including the 32 kb first intron, and ~77 kb of 5' and ~ 99 kb of 3' DNA flanking the gene, into which the coding sequence for enhanced green fluorescent protein (EGFP) has been inserted at the Kit initiation codon; a single Frt site remains associated with the EGFP insert. Neonatal mice exhibit intense skin fluorescence; gross UV examination and immunohistologic analysis for EGFP reveals adult EGFP expression in organs and specific cell types consistent with the known KIT expression pattern. Coexpression of EGFP and of KIT from the endogenous gene have been demonstrated in cell groups, although not always in the same individual cells, of the heart. (J:144922)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:144922 Tallini YN, et al., c-kit expression identifies cardiovascular precursors in the neonatal heart. Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1808-13
All:	5 reference(s)