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Gene Expression Data
Assay Details
Assay
Reference: J:143967 Biskup S, et al., Dynamic and redundant regulation of LRRK2 and LRRK1 expression. BMC Neurosci. 2007;8:102
Assay type: Western blot
MGI Accession ID: MGI:3834938
Gene symbol: Lrrk2
Gene name: leucine-rich repeat kinase 2
Antibody: anti-LRRK2 JH5514
Detection system: Secondary antibody
Assay notes: Whole mouse brain was mechanically homogenized in either PBS (low salt buffer), high salt buffer (PBS supplemented to 600 mM NaCl), 1% Triton X-100 (in PBS), 1% SDS (in PBS) or RIPA (1% SDS, 1% sodium deoxycholate, 1% NP-40). All buffers contained complete protease inhibitors.
Results
Image: 1A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 280 kDa Genetic BackgroundMutant Allele(s)Sex
KO/Low Salt P TS28: brain Absent involves: 129S/SvEv * C57BL/6 Lrrk2tm1Tmd/Lrrk2tm1TmdNot Specified
WT/Low Salt P TS28: brain Present Not Specified Not Specified
WT/High Salt P TS28: brain Present Not Specified Not Specified
WT/1% Triton P TS28: brain Present Not Specified Not Specified
WT/1% SDS P TS28: brain Present Not Specified Not Specified
WT/ RIPA P TS28: brain Present Not Specified Not Specified
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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