Gene Expression Data
Assay Details
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Assay
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Reference:
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J:121900
Sasaki M, et al., Disruption of the mouse protein Ser/Thr phosphatase 2Cbeta gene leads to early pre-implantation lethality. Mech Dev. 2007 Jul;124(6):489-99
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3834118
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Gene symbol:
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Ppm1b
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Gene name:
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protein phosphatase 1B, magnesium dependent, beta isoform
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Antibody:
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anti-PP2Cbeta
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Fixative is 3.7%
paraformaldehyde.
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Results
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Specimen
1A a:
postnatal
(more  )
Specimen
1A a:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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postnatal
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatozoon |
Present |
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1A a
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Expression is in the midpiece of spermatozoa.
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Specimen
1A e:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Ppm1btm1Tamu/Ppm1b+
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Age:
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postnatal
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatozoon |
Present |
Regionally restricted |
1A e
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Expression is in the midpiece of spermatozoa.
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Specimen
1B a:
postnatal
(more )
Note: MII oocytes.
Specimen
1B a:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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MII oocytes.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
Regionally restricted |
1B a
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Expression is in the spindle complex and cytoplasm of MII oocytes.
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Specimen
1B e:
postnatal; Ppm1btm1Tamu/Ppm1b +
(more )
Note: MII oocytes.
Specimen
1B e:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Ppm1btm1Tamu/Ppm1b+
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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MII oocytes.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
Regionally restricted |
1B e
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Expression is in the spindle complex and cytoplasm of MII oocytes.
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Specimen
1C a:
embryonic day 0.5
(more )
Note: Zygote.
Specimen
1C a:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Zygote.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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1C a
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Expression is in the cytoplasm, with some spots across the nucleus.
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Specimen
1C e:
embryonic day 1.0
(more )
Note: Early 2-cell embryo.
Specimen
1C e:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Early 2-cell embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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1C e
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Expression is in the cytoplasm, with some spots across the nucleus.
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Specimen
1C i:
embryonic day 1.5
(more )
Note: Late 2-cell embryo.
Specimen
1C i:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 1.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Late 2-cell embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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1C i
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Expression is in the cytoplasm, with some spots across the nucleus.
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Specimen
1C m:
embryonic day 2.0
(more )
Note: 4-cell embryo.
Specimen
1C m:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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4-cell embryo.
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Specimen
1C q:
embryonic day 2.5
(more )
Note: 8-cell embryo.
Specimen
1C q:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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8-cell embryo.
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Specimen
1C u:
embryonic day 4.0
(more )
Note: Blastocyst.
Specimen
1C u:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 4.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Blastocyst.
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