Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	En2tm6Alj
Name:	engrailed 2; targeted mutation 6, Alexandra L Joyner
MGI ID:	MGI:3831084
Synonyms:	En2flox, En2lox
Gene:	En2  Location: Chr5:28370692-28377164 bp, + strand  Genetic Position: Chr5, 13.94 cM
Alliance:	En2tm6Alj page

Germline Transmission:	Earliest citation of germline transmission: J:156169
Parent Cell Line:	W4 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Conditional ready, No functional change) Mutation:    Insertion   Mutation details: The targeting construct contains a 3.1 kb En2 5' fragment, an Frt-flanked PGK-neo cassette followed by a LoxP site in the En2 intron and a 4.8 kb En2 genomic fragment containing a LoxP site in the XbaI site of the 3' UTR. Flpe-mediated recombination deleted the PGK-neo, producing an En2 allele with a floxed region. (J:156169)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any En2 Mutation:	104 strains or lines available

Original:	J:156169 Cheng Y, et al., The Engrailed homeobox genes determine the different foliation patterns in the vermis and hemispheres of the mammalian cerebellum. Development. 2010 Feb;137(3):519-29
All:	3 reference(s)