Probe:	Prl7c1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Analysis was performed in three mouse strain backgrounds (CD-1 or Swiss, C57BL/6 and 129/SvJ). The expression patterns were generally consistent across the three strains and data are shown for the CD-1 background.

Structure	Level	Pattern	Image	Note
TS13: ectoplacental cone	Present	Regionally restricted	3_Prl7c1 , 3_Prl7c1 high mag.	Expression is in the center of the cone and not in parietal trophoblast giant cells.
TS28: placenta	Present	Regionally restricted	3_Prl7c1 , 3_Prl7c1 high mag.	Expression is in the center of the cone and not in parietal trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS13: ectoplacental cone	Present		Additional File Prl7c1_A E8.5
TS13: extraembryonic component	Present		Additional File Prl7c1_A E8.5
TS28: placenta	Present		Additional File Prl7c1_A E8.5

Structure	Level	Pattern	Image	Note
TS22: extraembryonic component	Present	Regionally restricted	Additional File Prl7c1_A E14.5	Expression is in glycogen trophoblast cells and is absent in the chorion or in syncytiotrophoblast of the labyrinth layer.
TS28: placenta	Present	Regionally restricted	Additional File Prl7c1_A E14.5	Expression is in glycogen trophoblast cells and is absent in the chorion or in syncytiotrophoblast of the labyrinth layer.