Xist RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:99399 Sado T, et al., Tsix Silences Xist through Modification of Chromatin Structure. Dev Cell. 2005 Jul;9(1):159-165
Assay type:	RT-PCR
MGI Accession ID:	MGI:3805508
Gene symbol:	Xist
Gene name:	inactive X specific transcripts

Probe:	Xist2281F, IVSR
Assay notes:	The primers used in this assay amplify the chimeric transcript.

Results

Image: 1A Xist^1lox

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
emb +	E13.5 (a)	TS21: embryo	Absent	1 µg; total RNA	Not Specified		Female
emb -	Control
ysm +	E13.5 (a)	TS21: yolk sac mesenchyme	Absent	1 µg; total RNA	Not Specified		Female
ysm -	Control
ve +	E13.5 (a)	TS21: yolk sac visceral endoderm	Absent	1 µg; total RNA	Not Specified		Female
ve -	Control
emb +	E13.5 (a)	TS21: embryo	Present	1 µg; total RNA	involves: 129S4/SvJae	Tsix^tm1Enl/Tsix⁺, Xist^tm1Sado/Xist⁺	Female
emb -	Control
ysm +	E13.5 (a)	TS21: yolk sac mesenchyme	Present	1 µg; total RNA	involves: 129S4/SvJae	Tsix^tm1Enl/Tsix⁺, Xist^tm1Sado/Xist⁺	Female
ysm -	Control
ve +	E13.5 (a)	TS21: yolk sac visceral endoderm	Present	1 µg; total RNA	involves: 129S4/SvJae	Tsix^tm1Enl/Tsix⁺, Xist^tm1Sado/Xist⁺	Female
ve -	Control
emb +	E13.5 (a)	TS21: embryo	Absent	1 µg; total RNA	Not Specified		Male
emb -	Control
ysm +	E13.5 (a)	TS21: yolk sac mesenchyme	Absent	1 µg; total RNA	Not Specified		Male
ysm -	Control
ve +	E13.5 (a)	TS21: yolk sac visceral endoderm	Absent	1 µg; total RNA	Not Specified		Male
ve -	Control
emb +	E13.5 (a)	TS21: embryo	Present	1 µg; total RNA	involves: 129S4/SvJae	Tsix^tm1Enl Xist^tm1Sado/Y	Male
emb -	Control
ysm +	E13.5 (a)	TS21: yolk sac mesenchyme	Present	1 µg; total RNA	involves: 129S4/SvJae	Tsix^tm1Enl Xist^tm1Sado/Y	Male
ysm -	Control
ve +	E13.5 (a)	TS21: yolk sac visceral endoderm	Present	1 µg; total RNA	involves: 129S4/SvJae	Tsix^tm1Enl Xist^tm1Sado/Y	Male
ve -	Control

Notes:
(a) Age of embryo at noon of plug day not specified in reference.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/14/2024 MGI 6.23