Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Fabp1-GH1)7Bir
Name:	transgene insertion 7, Edward H Birkenmeier
MGI ID:	MGI:3800327
Synonyms:	L-FABP(-596 to +21)/hGH transgene, short promoter L-FABP-hGH transgene, Tg(L-FABP, S-GH)7Bir
Transgene:	Tg(Fabp1-GH1)7Bir  Location: unknown
Alliance:	Tg(Fabp1-GH1)7Bir page

Strain of Origin:

(C57BL/6J x LT/Sv)F1

Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence) Mutation:    Insertion   Tg(Fabp1-GH1)7Bir expresses 1 gene Transgene expresses: Organism Expressed Gene Homolog in Mouse Note human GH1 (2688) Gh  (MGI:95707)   Mutation details: This transgene comprises a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -596 to +21 from the rat liver fatty-acid binding protein gene. Mice of this line (from founder G0#13) have ~500 transgene copies per haploid genome, as measured by densitometric analysis of Southern blots. Expression of human growth hormone mRNA, determined by dot-blot analysis, correlates generally with that of endogenous mouse Fabp1 mRNA in liver and intestine, including a proximal-to-distal declining expression gradient in the small intestine (with somewhat higher hGH expression in the ileum); neither is expressed in several other tissues examined, but hGH, unlike Fabp1, is expressed in cecum, colon and kidneys of transgenic mice. This "ectopic" expression is suppressed by more 5' regulatory sequences present in similar transgenes with a longer Fabp1 promoter. (See Tg(Fabp1-GH1)5Bir) (J:109939)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:109939 Sweetser DA, et al., Mechanisms underlying generation of gradients in gene expression within the intestine: an analysis using transgenic mice containing fatty acid binding protein-human growth hormone fusion genes. Genes Dev. 1988 Oct;2(10):1318-32
All:	5 reference(s)