Tg(DBH-RET*M918T)CH7194Rpk
Transgene Detail
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| Symbol: |
Tg(DBH-RET*M918T)CH7194Rpk |
| Name: |
transgene insertion CH7194, Raj P Kapur |
| MGI ID: |
MGI:3767823 |
| Synonyms: |
DbetaH-RETMEN2B, `intermediate' |
| Transgene: |
Tg(DBH-RET*M918T)CH7194Rpk Location: unknown
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| Alliance: |
Tg(DBH-RET*M918T)CH7194Rpk page
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| Strain of Origin: |
(DBA x C57BL/6)F1
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(DBH-RET*M918T)CH7194Rpk expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
RET (5979) |
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Transgene codes for a protein with a methionine to threonine mutation. |
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Mutation details: A human cDNA encoding the short isoform (1072 amino acids) of RET was mutated by PCR to introduce the RET MEN2B mutation (Met918Thr). This mutated cDNA was inserted downstream of a human DBH promoter (5.8 kb). Between the transcriptional start site and the mutated cDNA, the construct contains the first intron of the rat preproinsulin gene (+13 +183 nt). A polyadenylation site from the murine protamine 1 gene was inserted at the 3' end. This transgenic line has a copy number of 70.
(J:108163)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:108163 Sweetser DA, et al., Ganglioneuromas and renal anomalies are induced by activated RET(MEN2B) in transgenic mice. Oncogene. 1999 Jan 28;18(4):877-86 |
| All: |
3 reference(s) |
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Analysis Tools
